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Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola
Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in Malaysia (Hsueh, 2011; Nghia et al., 2008). The isolation of UM 591 from a patient’s contact lens indicates the pathogenic potential of this dematiaceous fu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5274520/ https://www.ncbi.nlm.nih.gov/pubmed/28149676 http://dx.doi.org/10.7717/peerj.2841 |
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author | Looi, Hong Keat Toh, Yue Fen Yew, Su Mei Na, Shiang Ling Tan, Yung-Chie Chong, Pei-Sin Khoo, Jia-Shiun Yee, Wai-Yan Ng, Kee Peng Kuan, Chee Sian |
author_facet | Looi, Hong Keat Toh, Yue Fen Yew, Su Mei Na, Shiang Ling Tan, Yung-Chie Chong, Pei-Sin Khoo, Jia-Shiun Yee, Wai-Yan Ng, Kee Peng Kuan, Chee Sian |
author_sort | Looi, Hong Keat |
collection | PubMed |
description | Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in Malaysia (Hsueh, 2011; Nghia et al., 2008). The isolation of UM 591 from a patient’s contact lens indicates the pathogenic potential of this dematiaceous fungus in human. However, the underlying factors that contribute to the opportunistic cross-infection have not been fully studied. We employed genome sequencing and gene homology annotations in attempt to identify these factors in UM 591 using data obtained from publicly available bioinformatics databases. The assembly size of UM 591 genome is 41.8 Mbp, and a total of 13,531 (≥99 bp) genes have been predicted. UM 591 is enriched with genes that encode for glycoside hydrolases, carbohydrate esterases, auxiliary activity enzymes and cell wall degrading enzymes. Virulent genes comprising of CAZymes, peptidases, and hypervirulence-associated cutinases were found to be present in the fungal genome. Comparative analysis result shows that UM 591 possesses higher number of carbohydrate esterases family 10 (CE10) CAZymes compared to other species of fungi in this study, and these enzymes hydrolyses wide range of carbohydrate and non-carbohydrate substrates. Putative melanin, siderophore, ent-kaurene, and lycopene biosynthesis gene clusters are predicted, and these gene clusters denote that UM 591 are capable of protecting itself from the UV and chemical stresses, allowing it to adapt to different environment. Putative sterigmatocystin, HC-toxin, cercosporin, and gliotoxin biosynthesis gene cluster are predicted. This finding have highlighted the necrotrophic and invasive nature of UM 591. |
format | Online Article Text |
id | pubmed-5274520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52745202017-02-01 Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola Looi, Hong Keat Toh, Yue Fen Yew, Su Mei Na, Shiang Ling Tan, Yung-Chie Chong, Pei-Sin Khoo, Jia-Shiun Yee, Wai-Yan Ng, Kee Peng Kuan, Chee Sian PeerJ Genomics Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in Malaysia (Hsueh, 2011; Nghia et al., 2008). The isolation of UM 591 from a patient’s contact lens indicates the pathogenic potential of this dematiaceous fungus in human. However, the underlying factors that contribute to the opportunistic cross-infection have not been fully studied. We employed genome sequencing and gene homology annotations in attempt to identify these factors in UM 591 using data obtained from publicly available bioinformatics databases. The assembly size of UM 591 genome is 41.8 Mbp, and a total of 13,531 (≥99 bp) genes have been predicted. UM 591 is enriched with genes that encode for glycoside hydrolases, carbohydrate esterases, auxiliary activity enzymes and cell wall degrading enzymes. Virulent genes comprising of CAZymes, peptidases, and hypervirulence-associated cutinases were found to be present in the fungal genome. Comparative analysis result shows that UM 591 possesses higher number of carbohydrate esterases family 10 (CE10) CAZymes compared to other species of fungi in this study, and these enzymes hydrolyses wide range of carbohydrate and non-carbohydrate substrates. Putative melanin, siderophore, ent-kaurene, and lycopene biosynthesis gene clusters are predicted, and these gene clusters denote that UM 591 are capable of protecting itself from the UV and chemical stresses, allowing it to adapt to different environment. Putative sterigmatocystin, HC-toxin, cercosporin, and gliotoxin biosynthesis gene cluster are predicted. This finding have highlighted the necrotrophic and invasive nature of UM 591. PeerJ Inc. 2017-01-26 /pmc/articles/PMC5274520/ /pubmed/28149676 http://dx.doi.org/10.7717/peerj.2841 Text en ©2017 Looi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Genomics Looi, Hong Keat Toh, Yue Fen Yew, Su Mei Na, Shiang Ling Tan, Yung-Chie Chong, Pei-Sin Khoo, Jia-Shiun Yee, Wai-Yan Ng, Kee Peng Kuan, Chee Sian Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title | Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title_full | Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title_fullStr | Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title_full_unstemmed | Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title_short | Genomic insight into pathogenicity of dematiaceous fungus Corynespora cassiicola |
title_sort | genomic insight into pathogenicity of dematiaceous fungus corynespora cassiicola |
topic | Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5274520/ https://www.ncbi.nlm.nih.gov/pubmed/28149676 http://dx.doi.org/10.7717/peerj.2841 |
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