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Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress

Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of...

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Autores principales: Bai, Zhen-yu, Wang, Tong, Wu, Yin-huan, Wang, Ke, Liang, Qian-yu, Pan, Yuan-zhi, Jiang, Bei-bei, Zhang, Lei, Liu, Guang-li, Jia, Yin, Liu, Qing-lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278365/
https://www.ncbi.nlm.nih.gov/pubmed/28134322
http://dx.doi.org/10.1038/srep41700
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author Bai, Zhen-yu
Wang, Tong
Wu, Yin-huan
Wang, Ke
Liang, Qian-yu
Pan, Yuan-zhi
Jiang, Bei-bei
Zhang, Lei
Liu, Guang-li
Jia, Yin
Liu, Qing-lin
author_facet Bai, Zhen-yu
Wang, Tong
Wu, Yin-huan
Wang, Ke
Liang, Qian-yu
Pan, Yuan-zhi
Jiang, Bei-bei
Zhang, Lei
Liu, Guang-li
Jia, Yin
Liu, Qing-lin
author_sort Bai, Zhen-yu
collection PubMed
description Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of contigs, transcripts and unigenes were obtained. Among them, only 30,814 unigenes were annotated by comparing with the protein databases. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment. In KEGG pathway, the mechanism of drought resistance in Phormium tenax was explained from three aspects of metabolism and signaling of hormones, osmotic adjustment and reactive oxygen species metabolism. These results are helpful to understand the drought tolerance mechanism of Phormium tenax and will provide a precious genetic resource for drought-resistant vegetation breeding and research.
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spelling pubmed-52783652017-02-03 Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress Bai, Zhen-yu Wang, Tong Wu, Yin-huan Wang, Ke Liang, Qian-yu Pan, Yuan-zhi Jiang, Bei-bei Zhang, Lei Liu, Guang-li Jia, Yin Liu, Qing-lin Sci Rep Article Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of contigs, transcripts and unigenes were obtained. Among them, only 30,814 unigenes were annotated by comparing with the protein databases. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment. In KEGG pathway, the mechanism of drought resistance in Phormium tenax was explained from three aspects of metabolism and signaling of hormones, osmotic adjustment and reactive oxygen species metabolism. These results are helpful to understand the drought tolerance mechanism of Phormium tenax and will provide a precious genetic resource for drought-resistant vegetation breeding and research. Nature Publishing Group 2017-01-30 /pmc/articles/PMC5278365/ /pubmed/28134322 http://dx.doi.org/10.1038/srep41700 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bai, Zhen-yu
Wang, Tong
Wu, Yin-huan
Wang, Ke
Liang, Qian-yu
Pan, Yuan-zhi
Jiang, Bei-bei
Zhang, Lei
Liu, Guang-li
Jia, Yin
Liu, Qing-lin
Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title_full Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title_fullStr Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title_full_unstemmed Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title_short Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress
title_sort whole-transcriptome sequence analysis of differentially expressed genes in phormium tenax under drought stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278365/
https://www.ncbi.nlm.nih.gov/pubmed/28134322
http://dx.doi.org/10.1038/srep41700
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