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Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner

We describe highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. Culture substrates have been generally thought to exert a cell adhesion effect when they are precoated onto culture vessels. However, simple addition of laminin fragments...

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Detalles Bibliográficos
Autores principales: Miyazaki, Takamichi, Isobe, Takehisa, Nakatsuji, Norio, Suemori, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278368/
https://www.ncbi.nlm.nih.gov/pubmed/28134277
http://dx.doi.org/10.1038/srep41165