Cargando…

Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner

We describe highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. Culture substrates have been generally thought to exert a cell adhesion effect when they are precoated onto culture vessels. However, simple addition of laminin fragments...

Descripción completa

Detalles Bibliográficos
Autores principales:	Miyazaki, Takamichi, Isobe, Takehisa, Nakatsuji, Norio, Suemori, Hirofumi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278368/ https://www.ncbi.nlm.nih.gov/pubmed/28134277 http://dx.doi.org/10.1038/srep41165

Ejemplares similares

Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
por: Miyazaki, Takamichi, et al.
Publicado: (2012)

Erratum: Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
por: Miyazaki, Takamichi, et al.
Publicado: (2013)

Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture
por: Takeuchi, Hiroki, et al.
Publicado: (2014)

Embryonic Stem Cell Lines of Nonhuman Primates
por: Nakatsuji, Norio, et al.
Publicado: (2002)

Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment
por: Sugawara, Yumika, et al.
Publicado: (2019)

Overexpression of Nuclear Receptor 5A1 Induces and Maintains an Intermediate State of Conversion between Primed and Naive Pluripotency
por: Yamauchi, Kaori, et al.
Publicado: (2020)

In Vitro Germ Cell Differentiation from Cynomolgus Monkey Embryonic Stem Cells
por: Yamauchi, Kaori, et al.
Publicado: (2009)

Efficient integration of transgenes into a defined locus in human embryonic stem cells
por: Sakurai, Kenji, et al.
Publicado: (2010)

Long-term maintenance of human induced pluripotent stem cells by automated cell culture system
por: Konagaya, Shuhei, et al.
Publicado: (2015)

BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer’s disease models
por: Nishioka, Hisae, et al.
Publicado: (2016)

Dynamic Link between Histone H3 Acetylation and an Increase in the Functional Characteristics of Human ESC/iPSC-Derived Cardiomyocytes
por: Otsuji, Tomomi G., et al.
Publicado: (2012)

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
por: Ohta, Ryo, et al.
Publicado: (2016)

Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511
por: Iwamuro, Masaya, et al.
Publicado: (2022)

Daxx Inhibits HIV-1 Reverse Transcription and Uncoating in a SUMO-Dependent Manner
por: Maillet, Sarah, et al.
Publicado: (2020)

Laminin-221-derived recombinant fragment facilitates isolation of cultured skeletal myoblasts
por: Kihara, Yuki, et al.
Publicado: (2022)

Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures
por: Lam, Alan Tin-Lun, et al.
Publicado: (2015)

What Kind of Signaling Maintains Pluripotency and Viability in Human-Induced Pluripotent Stem Cells Cultured on Laminin-511 with Serum-Free Medium?
por: Nakashima, Yoshiki, et al.
Publicado: (2016)

Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells
por: Akopian, Veronika, et al.
Publicado: (2010)

Adhesion of platelets to laminin in the absence of activation
Publicado: (1984)

Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression
por: Uosaki, Hideki, et al.
Publicado: (2011)

The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells
por: Takase, Osamu, et al.
Publicado: (2013)

Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity
por: Aoki, Hiromasa, et al.
Publicado: (2020)

Laminin fragments conjugated with perlecan’s growth factor-binding domain differentiate human induced pluripotent stem cells into skin-derived precursor cells
por: Sugiyama-Nakagiri, Yoriko, et al.
Publicado: (2023)

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells
por: Nakashima, Yoshiki, et al.
Publicado: (2022)

Polymerized Laminin-332 Matrix Supports Rapid and Tight Adhesion of Keratinocytes, Suppressing Cell Migration
por: Kariya, Yoshinobu, et al.
Publicado: (2012)

A Novel Serum-Free Monolayer Culture for Orderly Hematopoietic Differentiation of Human Pluripotent Cells via Mesodermal Progenitors
por: Niwa, Akira, et al.
Publicado: (2011)

A circular uncoating mechanism
por: Wells, William A.
Publicado: (2004)

Revisiting HIV-1 uncoating
por: Arhel, Nathalie
Publicado: (2010)

α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal
por: Laperle, Alex, et al.
Publicado: (2015)

Endogenous muscle lectin inhibits myoblast adhesion to laminin
Publicado: (1991)

Adhesion, growth, and matrix production by fibroblasts on laminin substrates
Publicado: (1983)

Amino-terminal fragments of laminin γ2 chain retract vascular endothelial cells and increase vascular permeability
por: Sato, Hiroki, et al.
Publicado: (2014)

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
por: Hyvärinen, Tanja, et al.
Publicado: (2019)

Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast
por: Gropp, Michal, et al.
Publicado: (2022)

A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production
por: Otsuji, Tomomi G., et al.
Publicado: (2014)

A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production
por: Otsuji, Tomomi G., et al.
Publicado: (2014)

Expression of 32/67-kDa laminin receptor in laminin adhesion-selected human colon cancer cell lines.
por: Kim, W. H., et al.
Publicado: (1998)

Efficient Culturing and Genetic Manipulation of Human Pluripotent Stem Cells
por: Schinzel, Robert T., et al.
Publicado: (2011)

Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
por: Luo, Dandan, et al.
Publicado: (2018)

Biomechanical and adhesion comparison of linea alba prophylactic reinforcement with coated and uncoated three-dimensional T-shaped mesh in rabbits
por: Hernani, Bruno De Lucia, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_2m5thl072u8lhop56b4in2pc39