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CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast
Over-expression of a gene by increasing its copy number is often desirable in the model yeast Saccharomyces cerevisiae. It may facilitate elucidation of enzyme functions, and in cell factory design it is used to increase production of proteins and metabolites. Current methods are typically exploitin...
Autores principales: | Strucko, Tomas, Buron, Line Due, Jarczynska, Zofia Dorota, Nødvig, Christina Spuur, Mølgaard, Louise, Halkier, Barbara Ann, Mortensen, Uffe Hasbro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278378/ https://www.ncbi.nlm.nih.gov/pubmed/28134264 http://dx.doi.org/10.1038/srep41431 |
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