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Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions
Neural stem cells (NSCs) are defined by their dual ability to self-renew through mitotic cell division or differentiate into the varied neural cell types of the CNS. DISP3/PTCHD2 is a sterol-sensing domain-containing protein, highly expressed in neural tissues, whose expression is regulated by thyro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278513/ https://www.ncbi.nlm.nih.gov/pubmed/28134287 http://dx.doi.org/10.1038/srep41597 |
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author | Konířová, Jana Oltová, Jana Corlett, Alicia Kopycińska, Justyna Kolář, Michal Bartůněk, Petr Zíková, Martina |
author_facet | Konířová, Jana Oltová, Jana Corlett, Alicia Kopycińska, Justyna Kolář, Michal Bartůněk, Petr Zíková, Martina |
author_sort | Konířová, Jana |
collection | PubMed |
description | Neural stem cells (NSCs) are defined by their dual ability to self-renew through mitotic cell division or differentiate into the varied neural cell types of the CNS. DISP3/PTCHD2 is a sterol-sensing domain-containing protein, highly expressed in neural tissues, whose expression is regulated by thyroid hormone. In the present study, we used a mouse NSC line to investigate what effect DISP3 may have on the self-renewal and/or differentiation potential of the cells. We demonstrated that NSC differentiation triggered significant reduction in DISP3 expression in the resulting astrocytes, neurons and oligodendrocytes. Moreover, when DISP3 expression was disrupted, the NSC “stemness” was suppressed, leading to a larger population of cells undergoing spontaneous neuronal differentiation. Conversely, overexpression of DISP3 resulted in increased NSC proliferation. When NSCs were cultured under differentiation conditions, we observed that the lack of DISP3 augmented the number of NSCs differentiating into each of the neural cell lineages and that neuronal morphology was altered. In contrast, DISP3 overexpression resulted in impaired cell differentiation. Taken together, our findings imply that DISP3 may help dictate the NSC cell fate to either undergo self-renewal or switch to the terminal differentiation cell program. |
format | Online Article Text |
id | pubmed-5278513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52785132017-02-03 Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions Konířová, Jana Oltová, Jana Corlett, Alicia Kopycińska, Justyna Kolář, Michal Bartůněk, Petr Zíková, Martina Sci Rep Article Neural stem cells (NSCs) are defined by their dual ability to self-renew through mitotic cell division or differentiate into the varied neural cell types of the CNS. DISP3/PTCHD2 is a sterol-sensing domain-containing protein, highly expressed in neural tissues, whose expression is regulated by thyroid hormone. In the present study, we used a mouse NSC line to investigate what effect DISP3 may have on the self-renewal and/or differentiation potential of the cells. We demonstrated that NSC differentiation triggered significant reduction in DISP3 expression in the resulting astrocytes, neurons and oligodendrocytes. Moreover, when DISP3 expression was disrupted, the NSC “stemness” was suppressed, leading to a larger population of cells undergoing spontaneous neuronal differentiation. Conversely, overexpression of DISP3 resulted in increased NSC proliferation. When NSCs were cultured under differentiation conditions, we observed that the lack of DISP3 augmented the number of NSCs differentiating into each of the neural cell lineages and that neuronal morphology was altered. In contrast, DISP3 overexpression resulted in impaired cell differentiation. Taken together, our findings imply that DISP3 may help dictate the NSC cell fate to either undergo self-renewal or switch to the terminal differentiation cell program. Nature Publishing Group 2017-01-30 /pmc/articles/PMC5278513/ /pubmed/28134287 http://dx.doi.org/10.1038/srep41597 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Konířová, Jana Oltová, Jana Corlett, Alicia Kopycińska, Justyna Kolář, Michal Bartůněk, Petr Zíková, Martina Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title | Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title_full | Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title_fullStr | Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title_full_unstemmed | Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title_short | Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions |
title_sort | modulated disp3/ptchd2 expression influences neural stem cell fate decisions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278513/ https://www.ncbi.nlm.nih.gov/pubmed/28134287 http://dx.doi.org/10.1038/srep41597 |
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