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Silver nanoparticles can attenuate nitrative stress

We have reported previously that glucose availability can modify toxicity of silver nanoparticles (AgNPs) via elevation of antioxidant defence triggered by increased mitochondrial generation of reactive oxygen species. In this study, we examined the effect of glucose availability on the production o...

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Detalles Bibliográficos
Autores principales: Zuberek, Mariusz, Paciorek, Patrycja, Bartosz, Grzegorz, Grzelak, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279695/
https://www.ncbi.nlm.nih.gov/pubmed/28157664
http://dx.doi.org/10.1016/j.redox.2017.01.011
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author Zuberek, Mariusz
Paciorek, Patrycja
Bartosz, Grzegorz
Grzelak, Agnieszka
author_facet Zuberek, Mariusz
Paciorek, Patrycja
Bartosz, Grzegorz
Grzelak, Agnieszka
author_sort Zuberek, Mariusz
collection PubMed
description We have reported previously that glucose availability can modify toxicity of silver nanoparticles (AgNPs) via elevation of antioxidant defence triggered by increased mitochondrial generation of reactive oxygen species. In this study, we examined the effect of glucose availability on the production of reactive nitrogen species in HepG2 cells and modification of nitrative stress by AgNPs. We found that lowering the glucose concentration increased expression of genes coding for inducible nitric oxide syntheas, NOS2 and NOS2A resulting in enhanced production of nitric oxide. Surprisingly, AgNPs decreased the level of nitric oxide accelerated denitration of proteins nitrated by exogenous peroxynitrite in cells grown in the presence of lowered glucose concentration, apparently due to further induction of protective proteins.
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spelling pubmed-52796952017-02-08 Silver nanoparticles can attenuate nitrative stress Zuberek, Mariusz Paciorek, Patrycja Bartosz, Grzegorz Grzelak, Agnieszka Redox Biol Research Paper We have reported previously that glucose availability can modify toxicity of silver nanoparticles (AgNPs) via elevation of antioxidant defence triggered by increased mitochondrial generation of reactive oxygen species. In this study, we examined the effect of glucose availability on the production of reactive nitrogen species in HepG2 cells and modification of nitrative stress by AgNPs. We found that lowering the glucose concentration increased expression of genes coding for inducible nitric oxide syntheas, NOS2 and NOS2A resulting in enhanced production of nitric oxide. Surprisingly, AgNPs decreased the level of nitric oxide accelerated denitration of proteins nitrated by exogenous peroxynitrite in cells grown in the presence of lowered glucose concentration, apparently due to further induction of protective proteins. Elsevier 2017-01-24 /pmc/articles/PMC5279695/ /pubmed/28157664 http://dx.doi.org/10.1016/j.redox.2017.01.011 Text en © 2017 Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Zuberek, Mariusz
Paciorek, Patrycja
Bartosz, Grzegorz
Grzelak, Agnieszka
Silver nanoparticles can attenuate nitrative stress
title Silver nanoparticles can attenuate nitrative stress
title_full Silver nanoparticles can attenuate nitrative stress
title_fullStr Silver nanoparticles can attenuate nitrative stress
title_full_unstemmed Silver nanoparticles can attenuate nitrative stress
title_short Silver nanoparticles can attenuate nitrative stress
title_sort silver nanoparticles can attenuate nitrative stress
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279695/
https://www.ncbi.nlm.nih.gov/pubmed/28157664
http://dx.doi.org/10.1016/j.redox.2017.01.011
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