Using the Volta phase plate with defocus for cryo-EM single particle analysis

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybri...

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Detalles Bibliográficos
Autores principales: Danev, Radostin, Tegunov, Dimitry, Baumeister, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279940/
https://www.ncbi.nlm.nih.gov/pubmed/28109158
http://dx.doi.org/10.7554/eLife.23006
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author Danev, Radostin
Tegunov, Dimitry
Baumeister, Wolfgang
author_facet Danev, Radostin
Tegunov, Dimitry
Baumeister, Wolfgang
author_sort Danev, Radostin
collection PubMed
description Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å. DOI: http://dx.doi.org/10.7554/eLife.23006.001
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spelling pubmed-52799402017-02-01 Using the Volta phase plate with defocus for cryo-EM single particle analysis Danev, Radostin Tegunov, Dimitry Baumeister, Wolfgang eLife Biophysics and Structural Biology Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å. DOI: http://dx.doi.org/10.7554/eLife.23006.001 eLife Sciences Publications, Ltd 2017-01-21 /pmc/articles/PMC5279940/ /pubmed/28109158 http://dx.doi.org/10.7554/eLife.23006 Text en © 2017, Danev et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biophysics and Structural Biology
Danev, Radostin
Tegunov, Dimitry
Baumeister, Wolfgang
Using the Volta phase plate with defocus for cryo-EM single particle analysis
title Using the Volta phase plate with defocus for cryo-EM single particle analysis
title_full Using the Volta phase plate with defocus for cryo-EM single particle analysis
title_fullStr Using the Volta phase plate with defocus for cryo-EM single particle analysis
title_full_unstemmed Using the Volta phase plate with defocus for cryo-EM single particle analysis
title_short Using the Volta phase plate with defocus for cryo-EM single particle analysis
title_sort using the volta phase plate with defocus for cryo-em single particle analysis
topic Biophysics and Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279940/
https://www.ncbi.nlm.nih.gov/pubmed/28109158
http://dx.doi.org/10.7554/eLife.23006
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