Using the Volta phase plate with defocus for cryo-EM single particle analysis

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybri...

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Detalles Bibliográficos
Autores principales: Danev, Radostin, Tegunov, Dimitry, Baumeister, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279940/
https://www.ncbi.nlm.nih.gov/pubmed/28109158
http://dx.doi.org/10.7554/eLife.23006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5279940/
https://www.ncbi.nlm.nih.gov/pubmed/28109158
http://dx.doi.org/10.7554/eLife.23006

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