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Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate

Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC(0)), Salmonella e...

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Autores principales: Fongaro, Gislaine, García-González, María C., Hernández, Marta, Kunz, Airton, Barardi, Célia R. M., Rodríguez-Lázaro, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5281563/
https://www.ncbi.nlm.nih.gov/pubmed/28197137
http://dx.doi.org/10.3389/fmicb.2017.00074
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author Fongaro, Gislaine
García-González, María C.
Hernández, Marta
Kunz, Airton
Barardi, Célia R. M.
Rodríguez-Lázaro, David
author_facet Fongaro, Gislaine
García-González, María C.
Hernández, Marta
Kunz, Airton
Barardi, Célia R. M.
Rodríguez-Lázaro, David
author_sort Fongaro, Gislaine
collection PubMed
description Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC(0)), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC(0) and PhiX-174 in clay soil was significantly lower than in sandy soil (iT(90) values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12.040 ± 0.010 vs. 43.470 ± 1.300, respectively) and PhiX-174 showed faster percolation and leaching in sandy soil than clay soil (iT(90) values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (iT(90) values of 9.340 ± 0.200 vs. 6.620 ± 0.500 and 11.900 ± 0.900 vs. 10.750 ± 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recycling.
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spelling pubmed-52815632017-02-14 Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate Fongaro, Gislaine García-González, María C. Hernández, Marta Kunz, Airton Barardi, Célia R. M. Rodríguez-Lázaro, David Front Microbiol Microbiology Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC(0)), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC(0) and PhiX-174 in clay soil was significantly lower than in sandy soil (iT(90) values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12.040 ± 0.010 vs. 43.470 ± 1.300, respectively) and PhiX-174 showed faster percolation and leaching in sandy soil than clay soil (iT(90) values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (iT(90) values of 9.340 ± 0.200 vs. 6.620 ± 0.500 and 11.900 ± 0.900 vs. 10.750 ± 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recycling. Frontiers Media S.A. 2017-01-31 /pmc/articles/PMC5281563/ /pubmed/28197137 http://dx.doi.org/10.3389/fmicb.2017.00074 Text en Copyright © 2017 Fongaro, García-González, Hernández, Kunz, Barardi and Rodríguez-Lázaro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fongaro, Gislaine
García-González, María C.
Hernández, Marta
Kunz, Airton
Barardi, Célia R. M.
Rodríguez-Lázaro, David
Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title_full Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title_fullStr Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title_full_unstemmed Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title_short Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate
title_sort different behavior of enteric bacteria and viruses in clay and sandy soils after biofertilization with swine digestate
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5281563/
https://www.ncbi.nlm.nih.gov/pubmed/28197137
http://dx.doi.org/10.3389/fmicb.2017.00074
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