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Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis

Sound vibration (SV) is considered to be a mechanical stimulus which gives rise to various physiological and molecular changes in plants. Previously, we identified 17 SV-regulated genes (SRGs) which were up-regulated by SV treatments in Arabidopsis. Here, we analyzed the expression pattern of simila...

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Autores principales: Ghosh, Ritesh, Gururani, Mayank A., Ponpandian, Lakshmi N., Mishra, Ratnesh C., Park, Soo-Chul, Jeong, Mi-Jeong, Bae, Hanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5281610/
https://www.ncbi.nlm.nih.gov/pubmed/28197168
http://dx.doi.org/10.3389/fpls.2017.00100
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author Ghosh, Ritesh
Gururani, Mayank A.
Ponpandian, Lakshmi N.
Mishra, Ratnesh C.
Park, Soo-Chul
Jeong, Mi-Jeong
Bae, Hanhong
author_facet Ghosh, Ritesh
Gururani, Mayank A.
Ponpandian, Lakshmi N.
Mishra, Ratnesh C.
Park, Soo-Chul
Jeong, Mi-Jeong
Bae, Hanhong
author_sort Ghosh, Ritesh
collection PubMed
description Sound vibration (SV) is considered to be a mechanical stimulus which gives rise to various physiological and molecular changes in plants. Previously, we identified 17 SV-regulated genes (SRGs) which were up-regulated by SV treatments in Arabidopsis. Here, we analyzed the expression pattern of similar genes after an exposure of 500 Hertz at 80 decibels, for various time periods. Simultaneously, we confirmed the SV-mediated expression of these genes under lighted condition as many of them were reported to be dark-induced. For this, we designed an improved SV treatment chamber. Additionally, we checked the electrolyte leakage (EL), photosynthetic performance and expression of mechanosensitive (MS) ion channel genes after 5 days of SV treatment in the illuminated chamber. EL was higher, and the photosynthetic performance index was lower in the SV-treated plants compared to control. Seven out of the 13 MS ion channel genes were differentially expressed after the SV treatment. Simultaneously, we checked the touch-mediated expression pattern of 17 SRGs and 13 MS ion channel genes. The distinct expression pattern of 6 SRGs and 1 MS ion channel gene generate an idea that SV as a stimulus is different from touch. Developmental stage-specific expression profiling suggested that the majority of the SRGs were expressed spatiotemporally in different developmental stages of Arabidopsis, especially in imbibed seed, seedlings and leaves.
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spelling pubmed-52816102017-02-14 Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis Ghosh, Ritesh Gururani, Mayank A. Ponpandian, Lakshmi N. Mishra, Ratnesh C. Park, Soo-Chul Jeong, Mi-Jeong Bae, Hanhong Front Plant Sci Plant Science Sound vibration (SV) is considered to be a mechanical stimulus which gives rise to various physiological and molecular changes in plants. Previously, we identified 17 SV-regulated genes (SRGs) which were up-regulated by SV treatments in Arabidopsis. Here, we analyzed the expression pattern of similar genes after an exposure of 500 Hertz at 80 decibels, for various time periods. Simultaneously, we confirmed the SV-mediated expression of these genes under lighted condition as many of them were reported to be dark-induced. For this, we designed an improved SV treatment chamber. Additionally, we checked the electrolyte leakage (EL), photosynthetic performance and expression of mechanosensitive (MS) ion channel genes after 5 days of SV treatment in the illuminated chamber. EL was higher, and the photosynthetic performance index was lower in the SV-treated plants compared to control. Seven out of the 13 MS ion channel genes were differentially expressed after the SV treatment. Simultaneously, we checked the touch-mediated expression pattern of 17 SRGs and 13 MS ion channel genes. The distinct expression pattern of 6 SRGs and 1 MS ion channel gene generate an idea that SV as a stimulus is different from touch. Developmental stage-specific expression profiling suggested that the majority of the SRGs were expressed spatiotemporally in different developmental stages of Arabidopsis, especially in imbibed seed, seedlings and leaves. Frontiers Media S.A. 2017-01-31 /pmc/articles/PMC5281610/ /pubmed/28197168 http://dx.doi.org/10.3389/fpls.2017.00100 Text en Copyright © 2017 Ghosh, Gururani, Ponpandian, Mishra, Park, Jeong and Bae. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ghosh, Ritesh
Gururani, Mayank A.
Ponpandian, Lakshmi N.
Mishra, Ratnesh C.
Park, Soo-Chul
Jeong, Mi-Jeong
Bae, Hanhong
Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title_full Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title_fullStr Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title_full_unstemmed Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title_short Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis
title_sort expression analysis of sound vibration-regulated genes by touch treatment in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5281610/
https://www.ncbi.nlm.nih.gov/pubmed/28197168
http://dx.doi.org/10.3389/fpls.2017.00100
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