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Polymorphism of apyrimidinic DNA structures in the nucleosome
Huge amounts (>10,000/day) of apurinic/apyrimidinic (AP) sites are produced in genomes, but their structures in chromatin remain undetermined. We determined the crystal structure of the nucleosome containing AP-site analogs at two symmetric sites, which revealed structural polymorphism: one forms...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282573/ https://www.ncbi.nlm.nih.gov/pubmed/28139742 http://dx.doi.org/10.1038/srep41783 |
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author | Osakabe, Akihisa Arimura, Yasuhiro Matsumoto, Syota Horikoshi, Naoki Sugasawa, Kaoru Kurumizaka, Hitoshi |
author_facet | Osakabe, Akihisa Arimura, Yasuhiro Matsumoto, Syota Horikoshi, Naoki Sugasawa, Kaoru Kurumizaka, Hitoshi |
author_sort | Osakabe, Akihisa |
collection | PubMed |
description | Huge amounts (>10,000/day) of apurinic/apyrimidinic (AP) sites are produced in genomes, but their structures in chromatin remain undetermined. We determined the crystal structure of the nucleosome containing AP-site analogs at two symmetric sites, which revealed structural polymorphism: one forms an inchworm configuration without an empty space at the AP site, and the other forms a B-form-like structure with an empty space and the orphan base. This unexpected inchworm configuration of the AP site is important to understand the AP DNA repair mechanism, because it may not be recognized by the major AP-binding protein, APE1, during the base excision repair process. |
format | Online Article Text |
id | pubmed-5282573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52825732017-02-03 Polymorphism of apyrimidinic DNA structures in the nucleosome Osakabe, Akihisa Arimura, Yasuhiro Matsumoto, Syota Horikoshi, Naoki Sugasawa, Kaoru Kurumizaka, Hitoshi Sci Rep Article Huge amounts (>10,000/day) of apurinic/apyrimidinic (AP) sites are produced in genomes, but their structures in chromatin remain undetermined. We determined the crystal structure of the nucleosome containing AP-site analogs at two symmetric sites, which revealed structural polymorphism: one forms an inchworm configuration without an empty space at the AP site, and the other forms a B-form-like structure with an empty space and the orphan base. This unexpected inchworm configuration of the AP site is important to understand the AP DNA repair mechanism, because it may not be recognized by the major AP-binding protein, APE1, during the base excision repair process. Nature Publishing Group 2017-01-31 /pmc/articles/PMC5282573/ /pubmed/28139742 http://dx.doi.org/10.1038/srep41783 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Osakabe, Akihisa Arimura, Yasuhiro Matsumoto, Syota Horikoshi, Naoki Sugasawa, Kaoru Kurumizaka, Hitoshi Polymorphism of apyrimidinic DNA structures in the nucleosome |
title | Polymorphism of apyrimidinic DNA structures in the nucleosome |
title_full | Polymorphism of apyrimidinic DNA structures in the nucleosome |
title_fullStr | Polymorphism of apyrimidinic DNA structures in the nucleosome |
title_full_unstemmed | Polymorphism of apyrimidinic DNA structures in the nucleosome |
title_short | Polymorphism of apyrimidinic DNA structures in the nucleosome |
title_sort | polymorphism of apyrimidinic dna structures in the nucleosome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282573/ https://www.ncbi.nlm.nih.gov/pubmed/28139742 http://dx.doi.org/10.1038/srep41783 |
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