Cargando…

No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)

Cry1Ie protein derived from Bacillus thuringiensis (Bt) has been proposed as a promising candidate for the development of a new Bt-maize variety to control maize pests in China. We studied the response of the midgut bacterial community of Apis cerana cerana to Cry1Ie toxin under laboratory condition...

Descripción completa

Detalles Bibliográficos
Autores principales: Jia, Hui-Ru, Dai, Ping-Li, Geng, Li-Li, Jack, Cameron J., Li, Yun-He, Wu, Yan-Yan, Diao, Qing-Yun, Ellis, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282592/
https://www.ncbi.nlm.nih.gov/pubmed/28139751
http://dx.doi.org/10.1038/srep41688
_version_ 1782503352885575680
author Jia, Hui-Ru
Dai, Ping-Li
Geng, Li-Li
Jack, Cameron J.
Li, Yun-He
Wu, Yan-Yan
Diao, Qing-Yun
Ellis, James D.
author_facet Jia, Hui-Ru
Dai, Ping-Li
Geng, Li-Li
Jack, Cameron J.
Li, Yun-He
Wu, Yan-Yan
Diao, Qing-Yun
Ellis, James D.
author_sort Jia, Hui-Ru
collection PubMed
description Cry1Ie protein derived from Bacillus thuringiensis (Bt) has been proposed as a promising candidate for the development of a new Bt-maize variety to control maize pests in China. We studied the response of the midgut bacterial community of Apis cerana cerana to Cry1Ie toxin under laboratory conditions. Newly emerged bees were fed one of the following treatments for 15 and 30 days: three concentrations of Cry1Ie toxin (20 ng/mL, 200 ng/mL, and 20 μg/mL) in sugar syrup, pure sugar syrup as a negative control and 48 ng/mL imidacloprid as a positive control. The relative abundance of 16S rRNA genes was measured by Quantitative Polymerase Chain Reaction and no apparent differences were found among treatments for any of these counts at any time point. Furthermore, the midgut bacterial structure and compositions were determined using high-throughput sequencing targeting the V3-V4 regions of the 16S rDNA. All core honey bee intestinal bacterial genera such as Lactobacillus, Bifidobacterium, Snodgrassella, and Gilliamella were detected, and no significant changes were found in the species diversity and richness for any bacterial taxa among treatments at different time points. These results suggest that Cry1Ie toxin may not affect gut bacterial communities of Chinese honey bees.
format Online
Article
Text
id pubmed-5282592
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-52825922017-02-03 No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae) Jia, Hui-Ru Dai, Ping-Li Geng, Li-Li Jack, Cameron J. Li, Yun-He Wu, Yan-Yan Diao, Qing-Yun Ellis, James D. Sci Rep Article Cry1Ie protein derived from Bacillus thuringiensis (Bt) has been proposed as a promising candidate for the development of a new Bt-maize variety to control maize pests in China. We studied the response of the midgut bacterial community of Apis cerana cerana to Cry1Ie toxin under laboratory conditions. Newly emerged bees were fed one of the following treatments for 15 and 30 days: three concentrations of Cry1Ie toxin (20 ng/mL, 200 ng/mL, and 20 μg/mL) in sugar syrup, pure sugar syrup as a negative control and 48 ng/mL imidacloprid as a positive control. The relative abundance of 16S rRNA genes was measured by Quantitative Polymerase Chain Reaction and no apparent differences were found among treatments for any of these counts at any time point. Furthermore, the midgut bacterial structure and compositions were determined using high-throughput sequencing targeting the V3-V4 regions of the 16S rDNA. All core honey bee intestinal bacterial genera such as Lactobacillus, Bifidobacterium, Snodgrassella, and Gilliamella were detected, and no significant changes were found in the species diversity and richness for any bacterial taxa among treatments at different time points. These results suggest that Cry1Ie toxin may not affect gut bacterial communities of Chinese honey bees. Nature Publishing Group 2017-01-31 /pmc/articles/PMC5282592/ /pubmed/28139751 http://dx.doi.org/10.1038/srep41688 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jia, Hui-Ru
Dai, Ping-Li
Geng, Li-Li
Jack, Cameron J.
Li, Yun-He
Wu, Yan-Yan
Diao, Qing-Yun
Ellis, James D.
No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title_full No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title_fullStr No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title_full_unstemmed No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title_short No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)
title_sort no effect of bt cry1ie toxin on bacterial diversity in the midgut of the chinese honey bees, apis cerana cerana (hymenoptera, apidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282592/
https://www.ncbi.nlm.nih.gov/pubmed/28139751
http://dx.doi.org/10.1038/srep41688
work_keys_str_mv AT jiahuiru noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT daipingli noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT genglili noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT jackcameronj noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT liyunhe noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT wuyanyan noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT diaoqingyun noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae
AT ellisjamesd noeffectofbtcry1ietoxinonbacterialdiversityinthemidgutofthechinesehoneybeesapisceranaceranahymenopteraapidae