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Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection

Low erucic acid is a major breeding target to improve the edible oil quality in Brassica juncea. The single nucleotide polymorphism (SNP) in fatty acid elongase 1 (FAE1.1 and FAE1.2) gene was exploited to expedite the breeding program. The paralogs of FAE1 gene were sequenced from low erucic acid ge...

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Autores principales: Saini, Navinder, Singh, Naveen, Kumar, Anil, Vihan, Nitika, Yadav, Sangita, Vasudev, Sujata, Yadava, D.K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282766/
https://www.ncbi.nlm.nih.gov/pubmed/28163599
http://dx.doi.org/10.1270/jsbbs.16132
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author Saini, Navinder
Singh, Naveen
Kumar, Anil
Vihan, Nitika
Yadav, Sangita
Vasudev, Sujata
Yadava, D.K.
author_facet Saini, Navinder
Singh, Naveen
Kumar, Anil
Vihan, Nitika
Yadav, Sangita
Vasudev, Sujata
Yadava, D.K.
author_sort Saini, Navinder
collection PubMed
description Low erucic acid is a major breeding target to improve the edible oil quality in Brassica juncea. The single nucleotide polymorphism (SNP) in fatty acid elongase 1 (FAE1.1 and FAE1.2) gene was exploited to expedite the breeding program. The paralogs of FAE1 gene were sequenced from low erucic acid genotype Pusa Mustard 30 and SNPs were identified through homologous alignment with sequence downloaded from NCBI GenBank. Two SNPs in FAE1.1 at position 591 and 1265 and one in FAE1.2 at 237 were found polymorphic among low and high erucic acid genotypes. These SNPs either create or change the recognition site of restriction enzymes. Transition of a single nucleotide at position 591 and 1265 in FAE1.1, and at position 237 in FAE1.2, leads to a change in the recognition site of Hpy99I, BglII and MnlI restriction enzymes, respectively. Two CAPS markers for FAE1.1 and one for FAE1.2 were developed to differentiate low and high erucic acid genotypes. The efficiency of these CAPS markers was found 100 per cent when validated in Brassica juncea, and B. nigra genotypes and used in back-cross breeding. These CAPS markers will facilitate in marker-assisted selection for improvement of oil quality in Brassica juncea.
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spelling pubmed-52827662017-02-03 Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection Saini, Navinder Singh, Naveen Kumar, Anil Vihan, Nitika Yadav, Sangita Vasudev, Sujata Yadava, D.K. Breed Sci Research Paper Low erucic acid is a major breeding target to improve the edible oil quality in Brassica juncea. The single nucleotide polymorphism (SNP) in fatty acid elongase 1 (FAE1.1 and FAE1.2) gene was exploited to expedite the breeding program. The paralogs of FAE1 gene were sequenced from low erucic acid genotype Pusa Mustard 30 and SNPs were identified through homologous alignment with sequence downloaded from NCBI GenBank. Two SNPs in FAE1.1 at position 591 and 1265 and one in FAE1.2 at 237 were found polymorphic among low and high erucic acid genotypes. These SNPs either create or change the recognition site of restriction enzymes. Transition of a single nucleotide at position 591 and 1265 in FAE1.1, and at position 237 in FAE1.2, leads to a change in the recognition site of Hpy99I, BglII and MnlI restriction enzymes, respectively. Two CAPS markers for FAE1.1 and one for FAE1.2 were developed to differentiate low and high erucic acid genotypes. The efficiency of these CAPS markers was found 100 per cent when validated in Brassica juncea, and B. nigra genotypes and used in back-cross breeding. These CAPS markers will facilitate in marker-assisted selection for improvement of oil quality in Brassica juncea. Japanese Society of Breeding 2016-12 2016-12-07 /pmc/articles/PMC5282766/ /pubmed/28163599 http://dx.doi.org/10.1270/jsbbs.16132 Text en Copyright © 2016 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Saini, Navinder
Singh, Naveen
Kumar, Anil
Vihan, Nitika
Yadav, Sangita
Vasudev, Sujata
Yadava, D.K.
Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title_full Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title_fullStr Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title_full_unstemmed Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title_short Development and validation of functional CAPS markers for the FAE genes in Brassica juncea and their use in marker-assisted selection
title_sort development and validation of functional caps markers for the fae genes in brassica juncea and their use in marker-assisted selection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282766/
https://www.ncbi.nlm.nih.gov/pubmed/28163599
http://dx.doi.org/10.1270/jsbbs.16132
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