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Microbial production of vitamin B(12): a review and future perspectives

Vitamin B(12) is an essential vitamin that is widely used in medical and food industries. Vitamin B(12) biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. Rational strain engineering is dependent on efficient genetic tools and a detaile...

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Detalles Bibliográficos
Autores principales: Fang, Huan, Kang, Jie, Zhang, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282855/
https://www.ncbi.nlm.nih.gov/pubmed/28137297
http://dx.doi.org/10.1186/s12934-017-0631-y
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author Fang, Huan
Kang, Jie
Zhang, Dawei
author_facet Fang, Huan
Kang, Jie
Zhang, Dawei
author_sort Fang, Huan
collection PubMed
description Vitamin B(12) is an essential vitamin that is widely used in medical and food industries. Vitamin B(12) biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. Rational strain engineering is dependent on efficient genetic tools and a detailed knowledge of metabolic pathways, regulation of which can be applied to improve product yield. Recent advances in synthetic biology and metabolic engineering have been used to efficiently construct many microbial chemical factories. Many published reviews have probed the vitamin B(12) biosynthetic pathway. To maximize the potential of microbes for vitamin B(12) production, new strategies and tools are required. In this review, we provide a comprehensive understanding of advances in the microbial production of vitamin B(12), with a particular focus on establishing a heterologous host for the vitamin B(12) production, as well as on strategies and tools that have been applied to increase microbial cobalamin production. Several worthy strategies employed for other products are also included.
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spelling pubmed-52828552017-02-03 Microbial production of vitamin B(12): a review and future perspectives Fang, Huan Kang, Jie Zhang, Dawei Microb Cell Fact Review Vitamin B(12) is an essential vitamin that is widely used in medical and food industries. Vitamin B(12) biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. Rational strain engineering is dependent on efficient genetic tools and a detailed knowledge of metabolic pathways, regulation of which can be applied to improve product yield. Recent advances in synthetic biology and metabolic engineering have been used to efficiently construct many microbial chemical factories. Many published reviews have probed the vitamin B(12) biosynthetic pathway. To maximize the potential of microbes for vitamin B(12) production, new strategies and tools are required. In this review, we provide a comprehensive understanding of advances in the microbial production of vitamin B(12), with a particular focus on establishing a heterologous host for the vitamin B(12) production, as well as on strategies and tools that have been applied to increase microbial cobalamin production. Several worthy strategies employed for other products are also included. BioMed Central 2017-01-30 /pmc/articles/PMC5282855/ /pubmed/28137297 http://dx.doi.org/10.1186/s12934-017-0631-y Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Fang, Huan
Kang, Jie
Zhang, Dawei
Microbial production of vitamin B(12): a review and future perspectives
title Microbial production of vitamin B(12): a review and future perspectives
title_full Microbial production of vitamin B(12): a review and future perspectives
title_fullStr Microbial production of vitamin B(12): a review and future perspectives
title_full_unstemmed Microbial production of vitamin B(12): a review and future perspectives
title_short Microbial production of vitamin B(12): a review and future perspectives
title_sort microbial production of vitamin b(12): a review and future perspectives
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282855/
https://www.ncbi.nlm.nih.gov/pubmed/28137297
http://dx.doi.org/10.1186/s12934-017-0631-y
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