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Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies
PURPOSE: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. METHODS: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was us...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283079/ https://www.ncbi.nlm.nih.gov/pubmed/28129422 http://dx.doi.org/10.1167/iovs.16-20272 |
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author | Zhu, Hong Kochevar, Irene E. Behlau, Irmgard Zhao, Jie Wang, Fenghua Wang, Yucheng Sun, Xiaodong Hamblin, Michael R. Dai, Tianhong |
author_facet | Zhu, Hong Kochevar, Irene E. Behlau, Irmgard Zhao, Jie Wang, Fenghua Wang, Yucheng Sun, Xiaodong Hamblin, Michael R. Dai, Tianhong |
author_sort | Zhu, Hong |
collection | PubMed |
description | PURPOSE: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. METHODS: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU). Using the ex vivo and in vivo models, the effectiveness of aBL (415 nm) for the treatment of keratitis was evaluated as a function of radiant exposure when aBL was delivered at 6 or 24 hours after bacterial inoculation. The aBL exposures calculated to reach the retina were compared to the American National Standards Institute standards to estimate aBL retinal safety. RESULTS: Pseudomonas aeruginosa keratitis fully developed in both the ex vivo and in vivo models at 24 hours post inoculation. Bacterial luminescence in the infected corneas correlated linearly to CFU (R(2) = 0.921). Bacterial burden in the infected corneas was rapidly and significantly reduced (>2-log(10)) both ex vivo and in vivo after a single exposure of aBL. Recurrence of infection was observed in the aBL-treated mice at 24 hours after aBL exposure. The aBL toxicity to the retina is largely dependent on the aBL transmission of the cornea. CONCLUSIONS: Antimicrobial blue light is a potential alternative or adjunctive therapeutic for infectious keratitis. Further studies of corneal and retinal safety using large animal models, in which the ocular anatomies are similar to that of humans, are warranted. |
format | Online Article Text |
id | pubmed-5283079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-52830792017-02-01 Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies Zhu, Hong Kochevar, Irene E. Behlau, Irmgard Zhao, Jie Wang, Fenghua Wang, Yucheng Sun, Xiaodong Hamblin, Michael R. Dai, Tianhong Invest Ophthalmol Vis Sci Cornea PURPOSE: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. METHODS: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU). Using the ex vivo and in vivo models, the effectiveness of aBL (415 nm) for the treatment of keratitis was evaluated as a function of radiant exposure when aBL was delivered at 6 or 24 hours after bacterial inoculation. The aBL exposures calculated to reach the retina were compared to the American National Standards Institute standards to estimate aBL retinal safety. RESULTS: Pseudomonas aeruginosa keratitis fully developed in both the ex vivo and in vivo models at 24 hours post inoculation. Bacterial luminescence in the infected corneas correlated linearly to CFU (R(2) = 0.921). Bacterial burden in the infected corneas was rapidly and significantly reduced (>2-log(10)) both ex vivo and in vivo after a single exposure of aBL. Recurrence of infection was observed in the aBL-treated mice at 24 hours after aBL exposure. The aBL toxicity to the retina is largely dependent on the aBL transmission of the cornea. CONCLUSIONS: Antimicrobial blue light is a potential alternative or adjunctive therapeutic for infectious keratitis. Further studies of corneal and retinal safety using large animal models, in which the ocular anatomies are similar to that of humans, are warranted. The Association for Research in Vision and Ophthalmology 2017-01 /pmc/articles/PMC5283079/ /pubmed/28129422 http://dx.doi.org/10.1167/iovs.16-20272 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Cornea Zhu, Hong Kochevar, Irene E. Behlau, Irmgard Zhao, Jie Wang, Fenghua Wang, Yucheng Sun, Xiaodong Hamblin, Michael R. Dai, Tianhong Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title | Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title_full | Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title_fullStr | Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title_full_unstemmed | Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title_short | Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies |
title_sort | antimicrobial blue light therapy for infectious keratitis: ex vivo and in vivo studies |
topic | Cornea |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283079/ https://www.ncbi.nlm.nih.gov/pubmed/28129422 http://dx.doi.org/10.1167/iovs.16-20272 |
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