In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation

Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the n...

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Detalles Bibliográficos
Autores principales: Choi, Hyun Woo, Hong, Yean Ju, Kim, Jong Soo, Song, Hyuk, Cho, Ssang Gu, Bae, Hojae, Kim, Changsung, Byun, Sung June, Do, Jeong Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283667/
https://www.ncbi.nlm.nih.gov/pubmed/28141814
http://dx.doi.org/10.1371/journal.pone.0170735
Descripción
Sumario:Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of fetal brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell types whose differentiation protocols are not well established.

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