In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation

Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the n...

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Autores principales: Choi, Hyun Woo, Hong, Yean Ju, Kim, Jong Soo, Song, Hyuk, Cho, Ssang Gu, Bae, Hojae, Kim, Changsung, Byun, Sung June, Do, Jeong Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283667/
https://www.ncbi.nlm.nih.gov/pubmed/28141814
http://dx.doi.org/10.1371/journal.pone.0170735
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author Choi, Hyun Woo
Hong, Yean Ju
Kim, Jong Soo
Song, Hyuk
Cho, Ssang Gu
Bae, Hojae
Kim, Changsung
Byun, Sung June
Do, Jeong Tae
author_facet Choi, Hyun Woo
Hong, Yean Ju
Kim, Jong Soo
Song, Hyuk
Cho, Ssang Gu
Bae, Hojae
Kim, Changsung
Byun, Sung June
Do, Jeong Tae
author_sort Choi, Hyun Woo
collection PubMed
description Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of fetal brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell types whose differentiation protocols are not well established.
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spelling pubmed-52836672017-02-17 In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation Choi, Hyun Woo Hong, Yean Ju Kim, Jong Soo Song, Hyuk Cho, Ssang Gu Bae, Hojae Kim, Changsung Byun, Sung June Do, Jeong Tae PLoS One Research Article Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of fetal brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell types whose differentiation protocols are not well established. Public Library of Science 2017-01-31 /pmc/articles/PMC5283667/ /pubmed/28141814 http://dx.doi.org/10.1371/journal.pone.0170735 Text en © 2017 Choi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Choi, Hyun Woo
Hong, Yean Ju
Kim, Jong Soo
Song, Hyuk
Cho, Ssang Gu
Bae, Hojae
Kim, Changsung
Byun, Sung June
Do, Jeong Tae
In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title_full In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title_fullStr In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title_full_unstemmed In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title_short In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
title_sort in vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283667/
https://www.ncbi.nlm.nih.gov/pubmed/28141814
http://dx.doi.org/10.1371/journal.pone.0170735
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