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Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust

Puccinia horiana Hennings, the causal agent of chrysanthemum white rust, is a worldwide quarantine organism and one of the most important fungal pathogens of Chrysanthemum × morifolium cultivars, which are used for cut flowers and as potted plants in commercial production regions of the world. It wa...

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Autores principales: Torres, David Eduardo, Rojas-Martínez, Reyna Isabel, Zavaleta-Mejía, Emma, Guevara-Fefer, Patricia, Márquez-Guzmán, G. Judith, Pérez-Martínez, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283677/
https://www.ncbi.nlm.nih.gov/pubmed/28141830
http://dx.doi.org/10.1371/journal.pone.0170782
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author Torres, David Eduardo
Rojas-Martínez, Reyna Isabel
Zavaleta-Mejía, Emma
Guevara-Fefer, Patricia
Márquez-Guzmán, G. Judith
Pérez-Martínez, Carolina
author_facet Torres, David Eduardo
Rojas-Martínez, Reyna Isabel
Zavaleta-Mejía, Emma
Guevara-Fefer, Patricia
Márquez-Guzmán, G. Judith
Pérez-Martínez, Carolina
author_sort Torres, David Eduardo
collection PubMed
description Puccinia horiana Hennings, the causal agent of chrysanthemum white rust, is a worldwide quarantine organism and one of the most important fungal pathogens of Chrysanthemum × morifolium cultivars, which are used for cut flowers and as potted plants in commercial production regions of the world. It was previously reported to be controlled by Lecanicillium lecanii, Cladosporium sphaerospermum, C. uredinicola and Aphanocladium album, due to their antagonistic and hyperparasitic effects. We report novel antagonist species on Puccinia horiana. Fungi isolated from rust pustules in a commercial greenhouse from Villa Guerrero, México, were identified as Cladosporium cladosporioides and Cladosporium pseudocladosporioides based upon molecular analysis and morphological characters. The antagonism of C. cladosporioides and C. pseudocladosporioides on chrysanthemum white rust was studied using light and electron microscopy in vitro at the host/parasite interface. Cladosporium cladosporioides and C. pseudocladosporioides grew towards the white rust teliospores and colonized the sporogenous cells, but no direct penetration of teliospores was observed; however, the structure and cytoplasm of teliospores were altered. The two Cladosporium spp. were able to grow on media containing laminarin, but not when chitin was used as the sole carbon source; these results suggest that they are able to produce glucanases. Results from the study indicate that both Cladosporium species had potential as biological control agents of chrysanthemum white rust.
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spelling pubmed-52836772017-02-17 Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust Torres, David Eduardo Rojas-Martínez, Reyna Isabel Zavaleta-Mejía, Emma Guevara-Fefer, Patricia Márquez-Guzmán, G. Judith Pérez-Martínez, Carolina PLoS One Research Article Puccinia horiana Hennings, the causal agent of chrysanthemum white rust, is a worldwide quarantine organism and one of the most important fungal pathogens of Chrysanthemum × morifolium cultivars, which are used for cut flowers and as potted plants in commercial production regions of the world. It was previously reported to be controlled by Lecanicillium lecanii, Cladosporium sphaerospermum, C. uredinicola and Aphanocladium album, due to their antagonistic and hyperparasitic effects. We report novel antagonist species on Puccinia horiana. Fungi isolated from rust pustules in a commercial greenhouse from Villa Guerrero, México, were identified as Cladosporium cladosporioides and Cladosporium pseudocladosporioides based upon molecular analysis and morphological characters. The antagonism of C. cladosporioides and C. pseudocladosporioides on chrysanthemum white rust was studied using light and electron microscopy in vitro at the host/parasite interface. Cladosporium cladosporioides and C. pseudocladosporioides grew towards the white rust teliospores and colonized the sporogenous cells, but no direct penetration of teliospores was observed; however, the structure and cytoplasm of teliospores were altered. The two Cladosporium spp. were able to grow on media containing laminarin, but not when chitin was used as the sole carbon source; these results suggest that they are able to produce glucanases. Results from the study indicate that both Cladosporium species had potential as biological control agents of chrysanthemum white rust. Public Library of Science 2017-01-31 /pmc/articles/PMC5283677/ /pubmed/28141830 http://dx.doi.org/10.1371/journal.pone.0170782 Text en © 2017 Torres et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Torres, David Eduardo
Rojas-Martínez, Reyna Isabel
Zavaleta-Mejía, Emma
Guevara-Fefer, Patricia
Márquez-Guzmán, G. Judith
Pérez-Martínez, Carolina
Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title_full Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title_fullStr Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title_full_unstemmed Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title_short Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust
title_sort cladosporium cladosporioides and cladosporium pseudocladosporioides as potential new fungal antagonists of puccinia horiana henn., the causal agent of chrysanthemum white rust
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283677/
https://www.ncbi.nlm.nih.gov/pubmed/28141830
http://dx.doi.org/10.1371/journal.pone.0170782
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