Cargando…
Measuring high-sensitivity cardiac troponin T blood concentration in population surveys
INTRODUCTION: The blood test for high-sensitivity cardiac troponin T (HS-CTnT) has been proposed as a marker of cardiovascular risk in the general population, as it is associated with subsequent incidence of cardiovascular events and mortality. We aimed at evaluating the feasibility of HS-CTnT testi...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283724/ https://www.ncbi.nlm.nih.gov/pubmed/28141863 http://dx.doi.org/10.1371/journal.pone.0171242 |
_version_ | 1782503539313999872 |
---|---|
author | Lazzarino, Antonio Ivan Mindell, Jennifer S. |
author_facet | Lazzarino, Antonio Ivan Mindell, Jennifer S. |
author_sort | Lazzarino, Antonio Ivan |
collection | PubMed |
description | INTRODUCTION: The blood test for high-sensitivity cardiac troponin T (HS-CTnT) has been proposed as a marker of cardiovascular risk in the general population, as it is associated with subsequent incidence of cardiovascular events and mortality. We aimed at evaluating the feasibility of HS-CTnT testing within large nationally-representative population surveys in which blood samples are collected during household visits, shipped using the standard civil postal service, and then frozen for subsequent analyses. METHODS: The Health Survey for England (HSE) consists of a series of annual surveys beginning in 1991. It is designed to provide regular information on various aspects of the nation’s health and risk factors. We measured HS-CTnT in the blood of 200 people from the HSE 2016 wave, then froze and stored their blood samples at -40°C for 5–10 weeks, and then thawed and retested them to appreciate the extent of within-person agreement or test-retest reliability of the two measurements. RESULTS: The Cronbach's Alpha (Scale Reliability Coefficient) and the Interclass Correlation Coefficient (two-way mixed-effects model for consistency of agreement at individual level) were 0.97 (95%CI = 0.96–0.99) and 0.95 (95%CI = 0.94–0.96) respectively. The time delay from blood withdrawal to analysis and storage (1–4 days) did not affect the results, nor did the freezing time before the retest (5–10 weeks). CONCLUSION: The measurement of HS-CTnT plasma concentration within large nationally-representative surveys such as the Health Survey for England is feasible. |
format | Online Article Text |
id | pubmed-5283724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52837242017-02-17 Measuring high-sensitivity cardiac troponin T blood concentration in population surveys Lazzarino, Antonio Ivan Mindell, Jennifer S. PLoS One Research Article INTRODUCTION: The blood test for high-sensitivity cardiac troponin T (HS-CTnT) has been proposed as a marker of cardiovascular risk in the general population, as it is associated with subsequent incidence of cardiovascular events and mortality. We aimed at evaluating the feasibility of HS-CTnT testing within large nationally-representative population surveys in which blood samples are collected during household visits, shipped using the standard civil postal service, and then frozen for subsequent analyses. METHODS: The Health Survey for England (HSE) consists of a series of annual surveys beginning in 1991. It is designed to provide regular information on various aspects of the nation’s health and risk factors. We measured HS-CTnT in the blood of 200 people from the HSE 2016 wave, then froze and stored their blood samples at -40°C for 5–10 weeks, and then thawed and retested them to appreciate the extent of within-person agreement or test-retest reliability of the two measurements. RESULTS: The Cronbach's Alpha (Scale Reliability Coefficient) and the Interclass Correlation Coefficient (two-way mixed-effects model for consistency of agreement at individual level) were 0.97 (95%CI = 0.96–0.99) and 0.95 (95%CI = 0.94–0.96) respectively. The time delay from blood withdrawal to analysis and storage (1–4 days) did not affect the results, nor did the freezing time before the retest (5–10 weeks). CONCLUSION: The measurement of HS-CTnT plasma concentration within large nationally-representative surveys such as the Health Survey for England is feasible. Public Library of Science 2017-01-31 /pmc/articles/PMC5283724/ /pubmed/28141863 http://dx.doi.org/10.1371/journal.pone.0171242 Text en © 2017 Lazzarino, Mindell http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lazzarino, Antonio Ivan Mindell, Jennifer S. Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title | Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title_full | Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title_fullStr | Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title_full_unstemmed | Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title_short | Measuring high-sensitivity cardiac troponin T blood concentration in population surveys |
title_sort | measuring high-sensitivity cardiac troponin t blood concentration in population surveys |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283724/ https://www.ncbi.nlm.nih.gov/pubmed/28141863 http://dx.doi.org/10.1371/journal.pone.0171242 |
work_keys_str_mv | AT lazzarinoantonioivan measuringhighsensitivitycardiactroponintbloodconcentrationinpopulationsurveys AT mindelljennifers measuringhighsensitivitycardiactroponintbloodconcentrationinpopulationsurveys |