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Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering

INTRODUCTION: Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases. PURPOSE: The aim was to isolate and characterize oMSCs from bone marrow aspirates (...

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Autores principales: Sanjurjo-Rodríguez, Clara, Castro-Viñuelas, Rocío, Hermida-Gómez, Tamara, Fernández-Vázquez, Tania, Fuentes-Boquete, Isaac Manuel, de Toro-Santos, Francisco Javier, Díaz-Prado, Silvia María, Blanco-García, Francisco Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283731/
https://www.ncbi.nlm.nih.gov/pubmed/28141815
http://dx.doi.org/10.1371/journal.pone.0171231
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author Sanjurjo-Rodríguez, Clara
Castro-Viñuelas, Rocío
Hermida-Gómez, Tamara
Fernández-Vázquez, Tania
Fuentes-Boquete, Isaac Manuel
de Toro-Santos, Francisco Javier
Díaz-Prado, Silvia María
Blanco-García, Francisco Javier
author_facet Sanjurjo-Rodríguez, Clara
Castro-Viñuelas, Rocío
Hermida-Gómez, Tamara
Fernández-Vázquez, Tania
Fuentes-Boquete, Isaac Manuel
de Toro-Santos, Francisco Javier
Díaz-Prado, Silvia María
Blanco-García, Francisco Javier
author_sort Sanjurjo-Rodríguez, Clara
collection PubMed
description INTRODUCTION: Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases. PURPOSE: The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds. METHODS: Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale. RESULTS: oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model. CONCLUSIONS: The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies.
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spelling pubmed-52837312017-02-17 Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering Sanjurjo-Rodríguez, Clara Castro-Viñuelas, Rocío Hermida-Gómez, Tamara Fernández-Vázquez, Tania Fuentes-Boquete, Isaac Manuel de Toro-Santos, Francisco Javier Díaz-Prado, Silvia María Blanco-García, Francisco Javier PLoS One Research Article INTRODUCTION: Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases. PURPOSE: The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds. METHODS: Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale. RESULTS: oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model. CONCLUSIONS: The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies. Public Library of Science 2017-01-31 /pmc/articles/PMC5283731/ /pubmed/28141815 http://dx.doi.org/10.1371/journal.pone.0171231 Text en © 2017 Sanjurjo-Rodríguez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sanjurjo-Rodríguez, Clara
Castro-Viñuelas, Rocío
Hermida-Gómez, Tamara
Fernández-Vázquez, Tania
Fuentes-Boquete, Isaac Manuel
de Toro-Santos, Francisco Javier
Díaz-Prado, Silvia María
Blanco-García, Francisco Javier
Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title_full Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title_fullStr Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title_full_unstemmed Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title_short Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering
title_sort ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283731/
https://www.ncbi.nlm.nih.gov/pubmed/28141815
http://dx.doi.org/10.1371/journal.pone.0171231
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