Cargando…

Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice

Infants born before 29 weeks gestation incur a major risk of preterm encephalopathy and subependymal/intracerebral/intraventricular haemorrhage. In mice, an ontogenic window of haemorrhage risk was recorded up to 5 days after birth in serpine1 knock-out animals. Using proteome and transcriptome appr...

Descripción completa

Detalles Bibliográficos
Autores principales: Porte, Baptiste, Chatelain, Clémence, Hardouin, Julie, Derambure, Céline, Zerdoumi, Yasmine, Hauchecorne, Michèle, Dupré, Nicolas, Bekri, Soumeya, Gonzalez, Bruno, Marret, Stéphane, Cosette, Pascal, Leroux, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283732/
https://www.ncbi.nlm.nih.gov/pubmed/28141873
http://dx.doi.org/10.1371/journal.pone.0171048
_version_ 1782503541112307712
author Porte, Baptiste
Chatelain, Clémence
Hardouin, Julie
Derambure, Céline
Zerdoumi, Yasmine
Hauchecorne, Michèle
Dupré, Nicolas
Bekri, Soumeya
Gonzalez, Bruno
Marret, Stéphane
Cosette, Pascal
Leroux, Philippe
author_facet Porte, Baptiste
Chatelain, Clémence
Hardouin, Julie
Derambure, Céline
Zerdoumi, Yasmine
Hauchecorne, Michèle
Dupré, Nicolas
Bekri, Soumeya
Gonzalez, Bruno
Marret, Stéphane
Cosette, Pascal
Leroux, Philippe
author_sort Porte, Baptiste
collection PubMed
description Infants born before 29 weeks gestation incur a major risk of preterm encephalopathy and subependymal/intracerebral/intraventricular haemorrhage. In mice, an ontogenic window of haemorrhage risk was recorded up to 5 days after birth in serpine1 knock-out animals. Using proteome and transcriptome approaches in mouse forebrain microvessels, we previously described the remodelling of extracellular matrix and integrins likely strengthening the vascular wall between postnatal day 5 (P5) and P10. Haemorrhage is the ultimate outcome of vessel damage (i.e., during ischaemia), although discreet vessel insults may be involved in the aetiology of preterm encephalopathy. In this study, we examined proteins identified by mass spectrometry and segregating in gene ontology pathways in forebrain microvessels in P5, P10, and adult wild type mice. In parallel, comparative transcript levels were obtained using RNA hybridization microarrays and enriched biological pathways were extracted from genes exhibiting at least a two-fold change in expression. Five major biological functions were observed in those genes detected both as proteins and mRNA expression undergoing at least a two-fold change in expression in one or more age comparisons: energy metabolism, protein metabolism, antioxidant function, ion exchanges, and transport. Adult microvessels exhibited the highest protein and mRNA expression levels for a majority of genes. Energy metabolism–enriched gene ontology pathways pointed to the preferential occurrence of glycolysis in P5 microvessels cells versus P10 and adult preparations enriched in aerobic oxidative enzymes. Age-dependent levels of RNA coding transport proteins at the plasma membrane and mitochondria strengthened our findings based on protein data. The data suggest that immature microvessels have fewer energy supply alternatives to glycolysis than mature structures. In the context of high energy demand, this constraint might account for vascular damage and maintenance of the high bleeding occurrence in specific areas in immature brain.
format Online
Article
Text
id pubmed-5283732
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-52837322017-02-17 Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice Porte, Baptiste Chatelain, Clémence Hardouin, Julie Derambure, Céline Zerdoumi, Yasmine Hauchecorne, Michèle Dupré, Nicolas Bekri, Soumeya Gonzalez, Bruno Marret, Stéphane Cosette, Pascal Leroux, Philippe PLoS One Research Article Infants born before 29 weeks gestation incur a major risk of preterm encephalopathy and subependymal/intracerebral/intraventricular haemorrhage. In mice, an ontogenic window of haemorrhage risk was recorded up to 5 days after birth in serpine1 knock-out animals. Using proteome and transcriptome approaches in mouse forebrain microvessels, we previously described the remodelling of extracellular matrix and integrins likely strengthening the vascular wall between postnatal day 5 (P5) and P10. Haemorrhage is the ultimate outcome of vessel damage (i.e., during ischaemia), although discreet vessel insults may be involved in the aetiology of preterm encephalopathy. In this study, we examined proteins identified by mass spectrometry and segregating in gene ontology pathways in forebrain microvessels in P5, P10, and adult wild type mice. In parallel, comparative transcript levels were obtained using RNA hybridization microarrays and enriched biological pathways were extracted from genes exhibiting at least a two-fold change in expression. Five major biological functions were observed in those genes detected both as proteins and mRNA expression undergoing at least a two-fold change in expression in one or more age comparisons: energy metabolism, protein metabolism, antioxidant function, ion exchanges, and transport. Adult microvessels exhibited the highest protein and mRNA expression levels for a majority of genes. Energy metabolism–enriched gene ontology pathways pointed to the preferential occurrence of glycolysis in P5 microvessels cells versus P10 and adult preparations enriched in aerobic oxidative enzymes. Age-dependent levels of RNA coding transport proteins at the plasma membrane and mitochondria strengthened our findings based on protein data. The data suggest that immature microvessels have fewer energy supply alternatives to glycolysis than mature structures. In the context of high energy demand, this constraint might account for vascular damage and maintenance of the high bleeding occurrence in specific areas in immature brain. Public Library of Science 2017-01-31 /pmc/articles/PMC5283732/ /pubmed/28141873 http://dx.doi.org/10.1371/journal.pone.0171048 Text en © 2017 Porte et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Porte, Baptiste
Chatelain, Clémence
Hardouin, Julie
Derambure, Céline
Zerdoumi, Yasmine
Hauchecorne, Michèle
Dupré, Nicolas
Bekri, Soumeya
Gonzalez, Bruno
Marret, Stéphane
Cosette, Pascal
Leroux, Philippe
Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title_full Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title_fullStr Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title_full_unstemmed Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title_short Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
title_sort proteomic and transcriptomic study of brain microvessels in neonatal and adult mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5283732/
https://www.ncbi.nlm.nih.gov/pubmed/28141873
http://dx.doi.org/10.1371/journal.pone.0171048
work_keys_str_mv AT portebaptiste proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT chatelainclemence proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT hardouinjulie proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT derambureceline proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT zerdoumiyasmine proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT hauchecornemichele proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT duprenicolas proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT bekrisoumeya proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT gonzalezbruno proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT marretstephane proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT cosettepascal proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice
AT lerouxphilippe proteomicandtranscriptomicstudyofbrainmicrovesselsinneonatalandadultmice