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Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway

Morus alba L. (MAL) extract has been used in traditional medicine for its cardioprotective and antiplatelet effects, while another herbal remedy, Schisandra chinensis (SCC), has been reported to have anti-inflammatory and antioxidant properties. We evaluated underlying cellular changes exerted by ex...

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Autores principales: Kim, Dong-Seon, Irfan, Muhammad, Sung, Yoon-Young, Kim, Seung Hyung, Park, Sun Haeng, Choi, Young Hyun, Rhee, Man Hee, Kim, Ho Kyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286545/
https://www.ncbi.nlm.nih.gov/pubmed/28194217
http://dx.doi.org/10.1155/2017/7839658
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author Kim, Dong-Seon
Irfan, Muhammad
Sung, Yoon-Young
Kim, Seung Hyung
Park, Sun Haeng
Choi, Young Hyun
Rhee, Man Hee
Kim, Ho Kyoung
author_facet Kim, Dong-Seon
Irfan, Muhammad
Sung, Yoon-Young
Kim, Seung Hyung
Park, Sun Haeng
Choi, Young Hyun
Rhee, Man Hee
Kim, Ho Kyoung
author_sort Kim, Dong-Seon
collection PubMed
description Morus alba L. (MAL) extract has been used in traditional medicine for its cardioprotective and antiplatelet effects, while another herbal remedy, Schisandra chinensis (SCC), has been reported to have anti-inflammatory and antioxidant properties. We evaluated underlying cellular changes exerted by extracts of these plants on platelet function and effects of SCC + MAL on in vivo thrombus formation using AV shunt and tail thrombosis-length models in rats. In vitro platelet aggregation, granule secretion, and [Ca(2+)](i) release assays were carried out. The activation of integrin α(IIb)β(3) and phosphorylation of downstream signaling molecules, including MAPK and Akt, were investigated using cytometry and immunoblotting, respectively. Scanning electron microscopy (SEM) was used to evaluate changes in platelet shape and HPLC analysis was carried out to identify the marker compounds in SCC + MAL mixture. In vivo thrombus weight and average length of tail thrombosis were significantly decreased by SCC + MAL. In vitro platelet aggregation, granule secretion, [Ca(2+)](i) release, and integrin α(IIb)β(3) activation were notably inhibited. SCC + MAL markedly reduced the phosphorylation of MAPK pathway factors along with Akt. HPLC analysis identified four marker compounds: isoquercitrin, astragalin, schizandrol A, and gomisin A. The extracts exerted remarkable synergistic effects as natural antithrombotic and antiplatelet agent and a potent drug candidate for treating cardiovascular diseases.
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spelling pubmed-52865452017-02-13 Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway Kim, Dong-Seon Irfan, Muhammad Sung, Yoon-Young Kim, Seung Hyung Park, Sun Haeng Choi, Young Hyun Rhee, Man Hee Kim, Ho Kyoung Evid Based Complement Alternat Med Research Article Morus alba L. (MAL) extract has been used in traditional medicine for its cardioprotective and antiplatelet effects, while another herbal remedy, Schisandra chinensis (SCC), has been reported to have anti-inflammatory and antioxidant properties. We evaluated underlying cellular changes exerted by extracts of these plants on platelet function and effects of SCC + MAL on in vivo thrombus formation using AV shunt and tail thrombosis-length models in rats. In vitro platelet aggregation, granule secretion, and [Ca(2+)](i) release assays were carried out. The activation of integrin α(IIb)β(3) and phosphorylation of downstream signaling molecules, including MAPK and Akt, were investigated using cytometry and immunoblotting, respectively. Scanning electron microscopy (SEM) was used to evaluate changes in platelet shape and HPLC analysis was carried out to identify the marker compounds in SCC + MAL mixture. In vivo thrombus weight and average length of tail thrombosis were significantly decreased by SCC + MAL. In vitro platelet aggregation, granule secretion, [Ca(2+)](i) release, and integrin α(IIb)β(3) activation were notably inhibited. SCC + MAL markedly reduced the phosphorylation of MAPK pathway factors along with Akt. HPLC analysis identified four marker compounds: isoquercitrin, astragalin, schizandrol A, and gomisin A. The extracts exerted remarkable synergistic effects as natural antithrombotic and antiplatelet agent and a potent drug candidate for treating cardiovascular diseases. Hindawi Publishing Corporation 2017 2017-01-18 /pmc/articles/PMC5286545/ /pubmed/28194217 http://dx.doi.org/10.1155/2017/7839658 Text en Copyright © 2017 Dong-Seon Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Dong-Seon
Irfan, Muhammad
Sung, Yoon-Young
Kim, Seung Hyung
Park, Sun Haeng
Choi, Young Hyun
Rhee, Man Hee
Kim, Ho Kyoung
Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title_full Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title_fullStr Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title_full_unstemmed Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title_short Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
title_sort schisandra chinensis and morus alba synergistically inhibit in vivo thrombus formation and platelet aggregation by impairing the glycoprotein vi pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286545/
https://www.ncbi.nlm.nih.gov/pubmed/28194217
http://dx.doi.org/10.1155/2017/7839658
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