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Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress

BACKGROUND: The Curcuma longa L. (CLL) rhizome has long been used to treat patients with hepatic dysfunction. CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed...

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Autores principales: Lee, Geum-Hwa, Lee, Hwa-Young, Choi, Min-Kyung, Chung, Han-Wool, Kim, Seung-Wook, Chae, Han-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286822/
https://www.ncbi.nlm.nih.gov/pubmed/28143589
http://dx.doi.org/10.1186/s13104-017-2409-z
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author Lee, Geum-Hwa
Lee, Hwa-Young
Choi, Min-Kyung
Chung, Han-Wool
Kim, Seung-Wook
Chae, Han-Jung
author_facet Lee, Geum-Hwa
Lee, Hwa-Young
Choi, Min-Kyung
Chung, Han-Wool
Kim, Seung-Wook
Chae, Han-Jung
author_sort Lee, Geum-Hwa
collection PubMed
description BACKGROUND: The Curcuma longa L. (CLL) rhizome has long been used to treat patients with hepatic dysfunction. CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed to evaluate the hepatoprotective activity of CLL extract and its active component curcumin in an acute carbon tetrachloride (CCl(4))-induced liver stress model. METHODS: Acute hepatic stress was induced by a single intraperitoneal injection of CCl(4) (0.1 ml/kg body weight) in rats. CLL extract was administered once a day for 3 days at three dose levels (100, 200, and 300 mg/kg/day) and curcumin was administered once a day at the 200 mg/kg/day. We performed alanine transaminase (ALT) and aspartate transaminase (AST). activity analysis and also measured total lipid, triglyceride, and cholesterol levels, and lipid peroxidation. RESULTS: At 100 g CLL, the curcuminoid components curcumin (901.63 ± 5.37 mg/100 g), bis-demethoxycurcumin (108.28 ± 2.89 mg/100 g), and demethoxycurcumin (234.85 ± 1.85 mg/100 g) were quantified through high liquid chromatography analysis. In CCl(4)-treated rats, serum AST and ALT levels increased 2.1- and 1.2-fold compared with the control. AST but not ALT elevation induced by CCl(4) was significantly alleviated in CLL- and curcumin-treated rats. Peroxidation of membrane lipids in the liver was significantly prevented by CLL (100, 200, and 300 mg/kg/day) on tissue lipid peroxidation assay and immunostaining with anti-4HNE antibody. We found that CLL extract and curcumin exhibited significant protection against liver injury by improving hepatic superoxide dismutase (p < 0.05) and glutathione peroxidase activity, and glutathione content in the CCl(4)-treated group (p < 0.05), leading to a reduced lipid peroxidase level. CONCLUSION: Our data suggested that CLL extract and curcumin protect the liver from acute CCl(4)-induced injury in a rodent model by suppressing hepatic oxidative stress. Therefore, CLL extract and curcumin are potential therapeutic antioxidant agents against acute hepatotoxicity.
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spelling pubmed-52868222017-02-06 Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress Lee, Geum-Hwa Lee, Hwa-Young Choi, Min-Kyung Chung, Han-Wool Kim, Seung-Wook Chae, Han-Jung BMC Res Notes Research Article BACKGROUND: The Curcuma longa L. (CLL) rhizome has long been used to treat patients with hepatic dysfunction. CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed to evaluate the hepatoprotective activity of CLL extract and its active component curcumin in an acute carbon tetrachloride (CCl(4))-induced liver stress model. METHODS: Acute hepatic stress was induced by a single intraperitoneal injection of CCl(4) (0.1 ml/kg body weight) in rats. CLL extract was administered once a day for 3 days at three dose levels (100, 200, and 300 mg/kg/day) and curcumin was administered once a day at the 200 mg/kg/day. We performed alanine transaminase (ALT) and aspartate transaminase (AST). activity analysis and also measured total lipid, triglyceride, and cholesterol levels, and lipid peroxidation. RESULTS: At 100 g CLL, the curcuminoid components curcumin (901.63 ± 5.37 mg/100 g), bis-demethoxycurcumin (108.28 ± 2.89 mg/100 g), and demethoxycurcumin (234.85 ± 1.85 mg/100 g) were quantified through high liquid chromatography analysis. In CCl(4)-treated rats, serum AST and ALT levels increased 2.1- and 1.2-fold compared with the control. AST but not ALT elevation induced by CCl(4) was significantly alleviated in CLL- and curcumin-treated rats. Peroxidation of membrane lipids in the liver was significantly prevented by CLL (100, 200, and 300 mg/kg/day) on tissue lipid peroxidation assay and immunostaining with anti-4HNE antibody. We found that CLL extract and curcumin exhibited significant protection against liver injury by improving hepatic superoxide dismutase (p < 0.05) and glutathione peroxidase activity, and glutathione content in the CCl(4)-treated group (p < 0.05), leading to a reduced lipid peroxidase level. CONCLUSION: Our data suggested that CLL extract and curcumin protect the liver from acute CCl(4)-induced injury in a rodent model by suppressing hepatic oxidative stress. Therefore, CLL extract and curcumin are potential therapeutic antioxidant agents against acute hepatotoxicity. BioMed Central 2017-02-01 /pmc/articles/PMC5286822/ /pubmed/28143589 http://dx.doi.org/10.1186/s13104-017-2409-z Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lee, Geum-Hwa
Lee, Hwa-Young
Choi, Min-Kyung
Chung, Han-Wool
Kim, Seung-Wook
Chae, Han-Jung
Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title_full Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title_fullStr Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title_full_unstemmed Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title_short Protective effect of Curcuma longa L. extract on CCl(4)-induced acute hepatic stress
title_sort protective effect of curcuma longa l. extract on ccl(4)-induced acute hepatic stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286822/
https://www.ncbi.nlm.nih.gov/pubmed/28143589
http://dx.doi.org/10.1186/s13104-017-2409-z
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