Cargando…
Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipoten...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287115/ https://www.ncbi.nlm.nih.gov/pubmed/28115468 http://dx.doi.org/10.1101/gad.289397.116 |
_version_ | 1782504107787943936 |
---|---|
author | Ingouff, Mathieu Selles, Benjamin Michaud, Caroline Vu, Thiet M. Berger, Frédéric Schorn, Andrea J. Autran, Daphné Van Durme, Matthias Nowack, Moritz K. Martienssen, Robert A. Grimanelli, Daniel |
author_facet | Ingouff, Mathieu Selles, Benjamin Michaud, Caroline Vu, Thiet M. Berger, Frédéric Schorn, Andrea J. Autran, Daphné Van Durme, Matthias Nowack, Moritz K. Martienssen, Robert A. Grimanelli, Daniel |
author_sort | Ingouff, Mathieu |
collection | PubMed |
description | Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells. |
format | Online Article Text |
id | pubmed-5287115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-52871152017-07-01 Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM Ingouff, Mathieu Selles, Benjamin Michaud, Caroline Vu, Thiet M. Berger, Frédéric Schorn, Andrea J. Autran, Daphné Van Durme, Matthias Nowack, Moritz K. Martienssen, Robert A. Grimanelli, Daniel Genes Dev Resource/Methodology Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells. Cold Spring Harbor Laboratory Press 2017-01-01 /pmc/articles/PMC5287115/ /pubmed/28115468 http://dx.doi.org/10.1101/gad.289397.116 Text en © 2017 Ingouff et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Resource/Methodology Ingouff, Mathieu Selles, Benjamin Michaud, Caroline Vu, Thiet M. Berger, Frédéric Schorn, Andrea J. Autran, Daphné Van Durme, Matthias Nowack, Moritz K. Martienssen, Robert A. Grimanelli, Daniel Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title | Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title_full | Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title_fullStr | Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title_full_unstemmed | Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title_short | Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM |
title_sort | live-cell analysis of dna methylation during sexual reproduction in arabidopsis reveals context and sex-specific dynamics controlled by noncanonical rddm |
topic | Resource/Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287115/ https://www.ncbi.nlm.nih.gov/pubmed/28115468 http://dx.doi.org/10.1101/gad.289397.116 |
work_keys_str_mv | AT ingouffmathieu livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT sellesbenjamin livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT michaudcaroline livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT vuthietm livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT bergerfrederic livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT schornandreaj livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT autrandaphne livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT vandurmematthias livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT nowackmoritzk livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT martienssenroberta livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm AT grimanellidaniel livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm |