Cargando…

Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM

Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipoten...

Descripción completa

Detalles Bibliográficos
Autores principales: Ingouff, Mathieu, Selles, Benjamin, Michaud, Caroline, Vu, Thiet M., Berger, Frédéric, Schorn, Andrea J., Autran, Daphné, Van Durme, Matthias, Nowack, Moritz K., Martienssen, Robert A., Grimanelli, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287115/
https://www.ncbi.nlm.nih.gov/pubmed/28115468
http://dx.doi.org/10.1101/gad.289397.116
_version_ 1782504107787943936
author Ingouff, Mathieu
Selles, Benjamin
Michaud, Caroline
Vu, Thiet M.
Berger, Frédéric
Schorn, Andrea J.
Autran, Daphné
Van Durme, Matthias
Nowack, Moritz K.
Martienssen, Robert A.
Grimanelli, Daniel
author_facet Ingouff, Mathieu
Selles, Benjamin
Michaud, Caroline
Vu, Thiet M.
Berger, Frédéric
Schorn, Andrea J.
Autran, Daphné
Van Durme, Matthias
Nowack, Moritz K.
Martienssen, Robert A.
Grimanelli, Daniel
author_sort Ingouff, Mathieu
collection PubMed
description Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells.
format Online
Article
Text
id pubmed-5287115
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-52871152017-07-01 Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM Ingouff, Mathieu Selles, Benjamin Michaud, Caroline Vu, Thiet M. Berger, Frédéric Schorn, Andrea J. Autran, Daphné Van Durme, Matthias Nowack, Moritz K. Martienssen, Robert A. Grimanelli, Daniel Genes Dev Resource/Methodology Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells. Cold Spring Harbor Laboratory Press 2017-01-01 /pmc/articles/PMC5287115/ /pubmed/28115468 http://dx.doi.org/10.1101/gad.289397.116 Text en © 2017 Ingouff et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Resource/Methodology
Ingouff, Mathieu
Selles, Benjamin
Michaud, Caroline
Vu, Thiet M.
Berger, Frédéric
Schorn, Andrea J.
Autran, Daphné
Van Durme, Matthias
Nowack, Moritz K.
Martienssen, Robert A.
Grimanelli, Daniel
Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title_full Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title_fullStr Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title_full_unstemmed Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title_short Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM
title_sort live-cell analysis of dna methylation during sexual reproduction in arabidopsis reveals context and sex-specific dynamics controlled by noncanonical rddm
topic Resource/Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287115/
https://www.ncbi.nlm.nih.gov/pubmed/28115468
http://dx.doi.org/10.1101/gad.289397.116
work_keys_str_mv AT ingouffmathieu livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT sellesbenjamin livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT michaudcaroline livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT vuthietm livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT bergerfrederic livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT schornandreaj livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT autrandaphne livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT vandurmematthias livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT nowackmoritzk livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT martienssenroberta livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm
AT grimanellidaniel livecellanalysisofdnamethylationduringsexualreproductioninarabidopsisrevealscontextandsexspecificdynamicscontrolledbynoncanonicalrddm