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Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fr...

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Autores principales: Kwak, A-Min, Lee, In-Kyoung, Lee, Sang-Yeop, Yun, Bong-Sik, Kang, Hee-Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287170/
https://www.ncbi.nlm.nih.gov/pubmed/28154495
http://dx.doi.org/10.5941/MYCO.2016.44.4.338
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author Kwak, A-Min
Lee, In-Kyoung
Lee, Sang-Yeop
Yun, Bong-Sik
Kang, Hee-Wan
author_facet Kwak, A-Min
Lee, In-Kyoung
Lee, Sang-Yeop
Yun, Bong-Sik
Kang, Hee-Wan
author_sort Kwak, A-Min
collection PubMed
description The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.
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spelling pubmed-52871702017-02-02 Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses Kwak, A-Min Lee, In-Kyoung Lee, Sang-Yeop Yun, Bong-Sik Kang, Hee-Wan Mycobiology Research Note The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases. The Korean Society of Mycology 2016-12 2016-12-31 /pmc/articles/PMC5287170/ /pubmed/28154495 http://dx.doi.org/10.5941/MYCO.2016.44.4.338 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Kwak, A-Min
Lee, In-Kyoung
Lee, Sang-Yeop
Yun, Bong-Sik
Kang, Hee-Wan
Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title_full Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title_fullStr Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title_full_unstemmed Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title_short Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
title_sort oxalic acid from lentinula edodes culture filtrate: antimicrobial activity on phytopathogenic bacteria and qualitative and quantitative analyses
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287170/
https://www.ncbi.nlm.nih.gov/pubmed/28154495
http://dx.doi.org/10.5941/MYCO.2016.44.4.338
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