Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets

[Image: see text] Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (K(d)) for protein–peptide interactions can be inferred. With the current platform, four...

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Detalles Bibliográficos
Autores principales: Gielen, Fabrice, Butz, Maren, Rees, Eric J., Erdelyi, Miklos, Moschetti, Tommaso, Hyvönen, Marko, Edel, Joshua B., Kaminski, Clemens F., Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287478/
https://www.ncbi.nlm.nih.gov/pubmed/28192993
http://dx.doi.org/10.1021/acs.analchem.6b02528

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