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PCR Inhibition of a Quantitative PCR for Detection of Mycobacterium avium Subspecies Paratuberculosis DNA in Feces: Diagnostic Implications and Potential Solutions

Molecular tests such as polymerase chain reaction (PCR) are increasingly being applied for the diagnosis of Johne’s disease, a chronic intestinal infection of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). Feces, as the primary test sample, presents challenges in terms of...

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Detalles Bibliográficos
Autores principales: Acharya, Kamal R., Dhand, Navneet K., Whittington, Richard J., Plain, Karren M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288348/
https://www.ncbi.nlm.nih.gov/pubmed/28210245
http://dx.doi.org/10.3389/fmicb.2017.00115