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Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China

Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both bla(OXA-48) and bla(NDM-1) on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility te...

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Autores principales: Xie, Lianyan, Dou, Yi, Zhou, Kaixin, Chen, Yue, Han, Lizhong, Guo, Xiaokui, Sun, Jingyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288367/
https://www.ncbi.nlm.nih.gov/pubmed/28210248
http://dx.doi.org/10.3389/fmicb.2017.00133
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author Xie, Lianyan
Dou, Yi
Zhou, Kaixin
Chen, Yue
Han, Lizhong
Guo, Xiaokui
Sun, Jingyong
author_facet Xie, Lianyan
Dou, Yi
Zhou, Kaixin
Chen, Yue
Han, Lizhong
Guo, Xiaokui
Sun, Jingyong
author_sort Xie, Lianyan
collection PubMed
description Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both bla(OXA-48) and bla(NDM-1) on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring bla(OXA-48) and bla(NDM-1) were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes. Plasmid DNA was sequenced using Pacbio RS II and annotated using RAST. Results: K. pneumoniae RJ119, carrying both bla(OXA-48) and bla(NDM-1), was resistant to almost all carbapenems, cephalosporins, fluoroquinolone, and aminoglycosides and belonged to ST307. bla(OXA-48) was located on a 61,748-bp IncL/M conjugative plasmid, which displayed overall nucleotide identity (99%) to pKPN-E1-Nr.7. bla(NDM-1) was located on a 335,317-bp conjugative plasmid, which was a fusion of a bla(NDM-1)-harboring InA/C plasmid pNDM-US (140,825 bp, 99% identity) and an IncFIB plasmid pKPN-c22 (178,563 bp, 99% identity). The transconjugant RJ119-1 harboring bla(NDM-1) was susceptible to carbapenem, and there was an insertion of IS10 into the bla(NDM-1) gene. Conclusion: This is the first report of the coexistence of bla(OXA-48) and bla(NDM-1) in one K. pneumoniae clinical isolate in China. OXA-48 in RJ119 contributed to the majority to its high resistance to carbapenems, whereas NDM-1 remained unexpressed, most likely due to the insertion of IS10. Our results provide new insight for the relationship between genetic diagnosis and clinical treatment. They also indicate that increased surveillance of bla(OXA-48) is urgently needed in China.
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spelling pubmed-52883672017-02-16 Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China Xie, Lianyan Dou, Yi Zhou, Kaixin Chen, Yue Han, Lizhong Guo, Xiaokui Sun, Jingyong Front Microbiol Microbiology Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both bla(OXA-48) and bla(NDM-1) on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring bla(OXA-48) and bla(NDM-1) were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes. Plasmid DNA was sequenced using Pacbio RS II and annotated using RAST. Results: K. pneumoniae RJ119, carrying both bla(OXA-48) and bla(NDM-1), was resistant to almost all carbapenems, cephalosporins, fluoroquinolone, and aminoglycosides and belonged to ST307. bla(OXA-48) was located on a 61,748-bp IncL/M conjugative plasmid, which displayed overall nucleotide identity (99%) to pKPN-E1-Nr.7. bla(NDM-1) was located on a 335,317-bp conjugative plasmid, which was a fusion of a bla(NDM-1)-harboring InA/C plasmid pNDM-US (140,825 bp, 99% identity) and an IncFIB plasmid pKPN-c22 (178,563 bp, 99% identity). The transconjugant RJ119-1 harboring bla(NDM-1) was susceptible to carbapenem, and there was an insertion of IS10 into the bla(NDM-1) gene. Conclusion: This is the first report of the coexistence of bla(OXA-48) and bla(NDM-1) in one K. pneumoniae clinical isolate in China. OXA-48 in RJ119 contributed to the majority to its high resistance to carbapenems, whereas NDM-1 remained unexpressed, most likely due to the insertion of IS10. Our results provide new insight for the relationship between genetic diagnosis and clinical treatment. They also indicate that increased surveillance of bla(OXA-48) is urgently needed in China. Frontiers Media S.A. 2017-02-02 /pmc/articles/PMC5288367/ /pubmed/28210248 http://dx.doi.org/10.3389/fmicb.2017.00133 Text en Copyright © 2017 Xie, Dou, Zhou, Chen, Han, Guo and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xie, Lianyan
Dou, Yi
Zhou, Kaixin
Chen, Yue
Han, Lizhong
Guo, Xiaokui
Sun, Jingyong
Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title_full Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title_fullStr Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title_full_unstemmed Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title_short Coexistence of bla(OXA-48) and Truncated bla(NDM-1) on Different Plasmids in a Klebsiella pneumoniae Isolate in China
title_sort coexistence of bla(oxa-48) and truncated bla(ndm-1) on different plasmids in a klebsiella pneumoniae isolate in china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288367/
https://www.ncbi.nlm.nih.gov/pubmed/28210248
http://dx.doi.org/10.3389/fmicb.2017.00133
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