Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis

Real‐Time Quaking‐Induced Conversion (RT‐QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and...

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Autores principales: Groveman, Bradley R., Orrú, Christina D., Hughson, Andrew G., Bongianni, Matilde, Fiorini, Michele, Imperiale, Daniele, Ladogana, Anna, Pocchiari, Maurizio, Zanusso, Gianluigi, Caughey, Byron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Brief Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288466/
https://www.ncbi.nlm.nih.gov/pubmed/28168213
http://dx.doi.org/10.1002/acn3.378
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author Groveman, Bradley R.
Orrú, Christina D.
Hughson, Andrew G.
Bongianni, Matilde
Fiorini, Michele
Imperiale, Daniele
Ladogana, Anna
Pocchiari, Maurizio
Zanusso, Gianluigi
Caughey, Byron
author_facet Groveman, Bradley R.
Orrú, Christina D.
Hughson, Andrew G.
Bongianni, Matilde
Fiorini, Michele
Imperiale, Daniele
Ladogana, Anna
Pocchiari, Maurizio
Zanusso, Gianluigi
Caughey, Byron
author_sort Groveman, Bradley R.
collection PubMed
description Real‐Time Quaking‐Induced Conversion (RT‐QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and concurrent comparison of our improved RT‐QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2‐day reduction in average detection time, and 100% specificity.
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spelling pubmed-52884662017-02-06 Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis Groveman, Bradley R. Orrú, Christina D. Hughson, Andrew G. Bongianni, Matilde Fiorini, Michele Imperiale, Daniele Ladogana, Anna Pocchiari, Maurizio Zanusso, Gianluigi Caughey, Byron Ann Clin Transl Neurol Brief Communications Real‐Time Quaking‐Induced Conversion (RT‐QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and concurrent comparison of our improved RT‐QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2‐day reduction in average detection time, and 100% specificity. John Wiley and Sons Inc. 2016-12-27 /pmc/articles/PMC5288466/ /pubmed/28168213 http://dx.doi.org/10.1002/acn3.378 Text en Published 2016. This article is a U.S. Government work and is in the public domain in the USA. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Brief Communications
Groveman, Bradley R.
Orrú, Christina D.
Hughson, Andrew G.
Bongianni, Matilde
Fiorini, Michele
Imperiale, Daniele
Ladogana, Anna
Pocchiari, Maurizio
Zanusso, Gianluigi
Caughey, Byron
Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title_full Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title_fullStr Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title_full_unstemmed Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title_short Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis
title_sort extended and direct evaluation of rt‐quic assays for creutzfeldt‐jakob disease diagnosis
topic Brief Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288466/
https://www.ncbi.nlm.nih.gov/pubmed/28168213
http://dx.doi.org/10.1002/acn3.378
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