Cargando…
Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels
Aim of the Study. To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action. Materials and Methods. ALF was induced in Wistar rats by administrating D-GalN (900 ...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288544/ https://www.ncbi.nlm.nih.gov/pubmed/28197212 http://dx.doi.org/10.1155/2017/9819350 |
_version_ | 1782504347160018944 |
---|---|
author | Wang, Minggang Shi, Qinglan Zhang, Rongzhen Qiu, Hua Mao, Dewen Long, Fuli |
author_facet | Wang, Minggang Shi, Qinglan Zhang, Rongzhen Qiu, Hua Mao, Dewen Long, Fuli |
author_sort | Wang, Minggang |
collection | PubMed |
description | Aim of the Study. To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action. Materials and Methods. ALF was induced in Wistar rats by administrating D-GalN (900 mg/kg) and lipopolysaccharide (10 μg/kg). After treatment with JDHY granules, the levels of blood alanine aminotransferase, aspartate aminotransferase, total bilirubin, and prothrombin time were determined. Proliferating cell nuclear antigen was detected by immunohistochemistry staining. The expression of interleukin-2 (IL-2) and toll-like receptor 4 (TLR4) was examined by fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results. JDHY treatment dramatically improved liver function and increased survival rates in an ALF model in rats. We observed a decrease in IL-2 and TLR4 expression following treatment with JDHY in liver cells from ALF rats using qRT-PCR and Western blot analysis. Conclusion. We hypothesize that the therapeutic potential of JDHY for treating ALF is due to its modulatory effect on the suppression of inflammation and by promoting hepatocyte regeneration. Our results contribute towards validation of the traditional use of JDHY in the treatment of liver disease. |
format | Online Article Text |
id | pubmed-5288544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52885442017-02-14 Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels Wang, Minggang Shi, Qinglan Zhang, Rongzhen Qiu, Hua Mao, Dewen Long, Fuli Evid Based Complement Alternat Med Research Article Aim of the Study. To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action. Materials and Methods. ALF was induced in Wistar rats by administrating D-GalN (900 mg/kg) and lipopolysaccharide (10 μg/kg). After treatment with JDHY granules, the levels of blood alanine aminotransferase, aspartate aminotransferase, total bilirubin, and prothrombin time were determined. Proliferating cell nuclear antigen was detected by immunohistochemistry staining. The expression of interleukin-2 (IL-2) and toll-like receptor 4 (TLR4) was examined by fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results. JDHY treatment dramatically improved liver function and increased survival rates in an ALF model in rats. We observed a decrease in IL-2 and TLR4 expression following treatment with JDHY in liver cells from ALF rats using qRT-PCR and Western blot analysis. Conclusion. We hypothesize that the therapeutic potential of JDHY for treating ALF is due to its modulatory effect on the suppression of inflammation and by promoting hepatocyte regeneration. Our results contribute towards validation of the traditional use of JDHY in the treatment of liver disease. Hindawi Publishing Corporation 2017 2017-01-19 /pmc/articles/PMC5288544/ /pubmed/28197212 http://dx.doi.org/10.1155/2017/9819350 Text en Copyright © 2017 Minggang Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Minggang Shi, Qinglan Zhang, Rongzhen Qiu, Hua Mao, Dewen Long, Fuli Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title | Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title_full | Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title_fullStr | Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title_full_unstemmed | Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title_short | Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels |
title_sort | herbal compound “jiedu huayu” reduces liver injury in rats via regulation of il-2, tlr4, and pcna expression levels |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288544/ https://www.ncbi.nlm.nih.gov/pubmed/28197212 http://dx.doi.org/10.1155/2017/9819350 |
work_keys_str_mv | AT wangminggang herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels AT shiqinglan herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels AT zhangrongzhen herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels AT qiuhua herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels AT maodewen herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels AT longfuli herbalcompoundjieduhuayureducesliverinjuryinratsviaregulationofil2tlr4andpcnaexpressionlevels |