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Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase

Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3′ overhangs of A, C, G, or T. Nucleotides were appended efficien...

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Detalles Bibliográficos
Autores principales:	Ohtsubo, Yoshiyuki, Nagata, Yuji, Tsuda, Masataka
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288710/ https://www.ncbi.nlm.nih.gov/pubmed/28150748 http://dx.doi.org/10.1038/srep41769

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