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Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens

Commensal bacteria co-exist on the mucosal surfaces of all vertebrates. The host’s mucosal immune system must tolerate commensals while fighting pathogens. One of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (Igs) across epithelial barr...

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Autores principales: Kelly, Cecelia, Takizawa, Fumio, Sunyer, J. Oriol, Salinas, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288726/
https://www.ncbi.nlm.nih.gov/pubmed/28150752
http://dx.doi.org/10.1038/srep41753
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author Kelly, Cecelia
Takizawa, Fumio
Sunyer, J. Oriol
Salinas, Irene
author_facet Kelly, Cecelia
Takizawa, Fumio
Sunyer, J. Oriol
Salinas, Irene
author_sort Kelly, Cecelia
collection PubMed
description Commensal bacteria co-exist on the mucosal surfaces of all vertebrates. The host’s mucosal immune system must tolerate commensals while fighting pathogens. One of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (Igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pIgR). Rainbow trout pIgR is known to transport IgT and IgM across epithelia. However, other biological functions for trout pIgR or trout secretory component (tSC) remain unknown. This study investigates the interaction of tSC with commensal bacteria, pathogenic bacteria and a fungal pathogen. Our results show that the majority of trout skin and gut bacteria are coated in vivo by tSC. In vitro, tSC present in mucus coats trout commensal isolates such as Microbacterium sp., Staphylococcus warneri, Flectobacillus major, Arthrobacter stackebrantii, and Flavobacterium sp. and the pathogens Vibrio anguillarum and Edwardsiella ictaluri with coating levels ranging from 8% to 70%. Moreover, we found that the majority of tSC is in free form in trout mucus and free tSC is able to directly bind bacteria. We propose that binding of free SC to commensal bacteria is a key and conserved mechanism for maintenance of microbial communities in vertebrate mucosal surfaces.
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spelling pubmed-52887262017-02-06 Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens Kelly, Cecelia Takizawa, Fumio Sunyer, J. Oriol Salinas, Irene Sci Rep Article Commensal bacteria co-exist on the mucosal surfaces of all vertebrates. The host’s mucosal immune system must tolerate commensals while fighting pathogens. One of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (Igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pIgR). Rainbow trout pIgR is known to transport IgT and IgM across epithelia. However, other biological functions for trout pIgR or trout secretory component (tSC) remain unknown. This study investigates the interaction of tSC with commensal bacteria, pathogenic bacteria and a fungal pathogen. Our results show that the majority of trout skin and gut bacteria are coated in vivo by tSC. In vitro, tSC present in mucus coats trout commensal isolates such as Microbacterium sp., Staphylococcus warneri, Flectobacillus major, Arthrobacter stackebrantii, and Flavobacterium sp. and the pathogens Vibrio anguillarum and Edwardsiella ictaluri with coating levels ranging from 8% to 70%. Moreover, we found that the majority of tSC is in free form in trout mucus and free tSC is able to directly bind bacteria. We propose that binding of free SC to commensal bacteria is a key and conserved mechanism for maintenance of microbial communities in vertebrate mucosal surfaces. Nature Publishing Group 2017-02-02 /pmc/articles/PMC5288726/ /pubmed/28150752 http://dx.doi.org/10.1038/srep41753 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kelly, Cecelia
Takizawa, Fumio
Sunyer, J. Oriol
Salinas, Irene
Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title_full Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title_fullStr Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title_full_unstemmed Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title_short Rainbow trout (Oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
title_sort rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288726/
https://www.ncbi.nlm.nih.gov/pubmed/28150752
http://dx.doi.org/10.1038/srep41753
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