Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

The alcohol oxidase 1 promoter (P(AOX1)) of Pichia pastoris is commonly used for high level expression of recombinant proteins. While the safety risk of methanol and tough process control for methanol induction usually cause problems especially in large-scale fermentation. By testing the functions o...

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Detalles Bibliográficos
Autores principales: Wang, Jinjia, Wang, Xiaolong, Shi, Lei, Qi, Fei, Zhang, Ping, Zhang, Yuanxing, Zhou, Xiangshan, Song, Zhiwei, Cai, Menghao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288789/
https://www.ncbi.nlm.nih.gov/pubmed/28150747
http://dx.doi.org/10.1038/srep41850

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288789/
https://www.ncbi.nlm.nih.gov/pubmed/28150747
http://dx.doi.org/10.1038/srep41850

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