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Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways

OBJECTIVE: The study was designed to investigate the ameliorative effect of Kolaviron (KV) on ischemic/reperfusion injury in experimental animal models. MATERIALS AND METHODS: Male Wistar rats were randomly divided into two groups: Group 1 received corn oil as a vehicle and rats in Group 2 were admi...

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Autores principales: Oyagbemi, Ademola Adetokunbo, Bester, Dirk, Esterhuyse, Johan, Farombi, Ebenezer Olatunde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ejmanager 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289087/
https://www.ncbi.nlm.nih.gov/pubmed/28163959
http://dx.doi.org/10.5455/jice.20160923100223
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author Oyagbemi, Ademola Adetokunbo
Bester, Dirk
Esterhuyse, Johan
Farombi, Ebenezer Olatunde
author_facet Oyagbemi, Ademola Adetokunbo
Bester, Dirk
Esterhuyse, Johan
Farombi, Ebenezer Olatunde
author_sort Oyagbemi, Ademola Adetokunbo
collection PubMed
description OBJECTIVE: The study was designed to investigate the ameliorative effect of Kolaviron (KV) on ischemic/reperfusion injury in experimental animal models. MATERIALS AND METHODS: Male Wistar rats were randomly divided into two groups: Group 1 received corn oil as a vehicle and rats in Group 2 were administered KV at 200 mg/kg for 4 weeks. The rats were fed with rat standard chow pellet and water administered ad libitum. After 4 weeks of KV administration, hearts were excised and mounted on the working heart perfusion system. Western blot analysis for protein expression was carried out on frozen heart samples. RESULTS: There was significant (P < 0.05) reduction in the activity of catalase, superoxide dismutase, and glutathione peroxidase with concomitant reduction in oxygen radical absorbance capacity in ischemic rat heart of control compared to group pre-treated with KV, respectively. Similarly, intracellular reactive oxygen species and malondialdehyde were significantly elevated in control compared to KV pre-treated rats. KV significantly increased total Akt/protein kinase B (PKB), phosphorylated Akt/PKB at serine 473 and also caused a significant reduction in p38 mitogen-activated protein kinase, Caspase 3, and cleaved poly adenosine diphosphate ribose polymerase. CONCLUSION: Taken together, KV offered significant cardioprotection via free radical scavenging activity and upregulation of pro-survival pathway.
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spelling pubmed-52890872017-02-03 Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways Oyagbemi, Ademola Adetokunbo Bester, Dirk Esterhuyse, Johan Farombi, Ebenezer Olatunde J Intercult Ethnopharmacol Original Research OBJECTIVE: The study was designed to investigate the ameliorative effect of Kolaviron (KV) on ischemic/reperfusion injury in experimental animal models. MATERIALS AND METHODS: Male Wistar rats were randomly divided into two groups: Group 1 received corn oil as a vehicle and rats in Group 2 were administered KV at 200 mg/kg for 4 weeks. The rats were fed with rat standard chow pellet and water administered ad libitum. After 4 weeks of KV administration, hearts were excised and mounted on the working heart perfusion system. Western blot analysis for protein expression was carried out on frozen heart samples. RESULTS: There was significant (P < 0.05) reduction in the activity of catalase, superoxide dismutase, and glutathione peroxidase with concomitant reduction in oxygen radical absorbance capacity in ischemic rat heart of control compared to group pre-treated with KV, respectively. Similarly, intracellular reactive oxygen species and malondialdehyde were significantly elevated in control compared to KV pre-treated rats. KV significantly increased total Akt/protein kinase B (PKB), phosphorylated Akt/PKB at serine 473 and also caused a significant reduction in p38 mitogen-activated protein kinase, Caspase 3, and cleaved poly adenosine diphosphate ribose polymerase. CONCLUSION: Taken together, KV offered significant cardioprotection via free radical scavenging activity and upregulation of pro-survival pathway. Ejmanager 2016-09-28 /pmc/articles/PMC5289087/ /pubmed/28163959 http://dx.doi.org/10.5455/jice.20160923100223 Text en Copyright: © EJManager http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Original Research
Oyagbemi, Ademola Adetokunbo
Bester, Dirk
Esterhuyse, Johan
Farombi, Ebenezer Olatunde
Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title_full Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title_fullStr Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title_full_unstemmed Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title_short Kolaviron, a biflavonoid of Garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
title_sort kolaviron, a biflavonoid of garcinia kola seed mitigates ischemic/reperfusion injury by modulation of pro-survival and apoptotic signaling pathways
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289087/
https://www.ncbi.nlm.nih.gov/pubmed/28163959
http://dx.doi.org/10.5455/jice.20160923100223
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