Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay

Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from w...

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Autores principales: OKUWA, Yuka, MIYAMATO-HAYASHI, Michiko, TANAKA, Takaichi, HAYAKAWA, Yuji, INOSHIMA, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289250/
https://www.ncbi.nlm.nih.gov/pubmed/27725415
http://dx.doi.org/10.1292/jvms.16-0328
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author OKUWA, Yuka
MIYAMATO-HAYASHI, Michiko
TANAKA, Takaichi
HAYAKAWA, Yuji
INOSHIMA, Yasuo
author_facet OKUWA, Yuka
MIYAMATO-HAYASHI, Michiko
TANAKA, Takaichi
HAYAKAWA, Yuji
INOSHIMA, Yasuo
author_sort OKUWA, Yuka
collection PubMed
description Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV.
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spelling pubmed-52892502017-02-08 Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay OKUWA, Yuka MIYAMATO-HAYASHI, Michiko TANAKA, Takaichi HAYAKAWA, Yuji INOSHIMA, Yasuo J Vet Med Sci Virology Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV. The Japanese Society of Veterinary Science 2016-10-03 2017-01 /pmc/articles/PMC5289250/ /pubmed/27725415 http://dx.doi.org/10.1292/jvms.16-0328 Text en ©2017 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
OKUWA, Yuka
MIYAMATO-HAYASHI, Michiko
TANAKA, Takaichi
HAYAKAWA, Yuji
INOSHIMA, Yasuo
Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title_full Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title_fullStr Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title_full_unstemmed Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title_short Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
title_sort simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289250/
https://www.ncbi.nlm.nih.gov/pubmed/27725415
http://dx.doi.org/10.1292/jvms.16-0328
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