Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens

We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subject...

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Autores principales: SUZUKI, Kazuya, OGUMA, Keisuke, SENTSUI, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289251/
https://www.ncbi.nlm.nih.gov/pubmed/27795464
http://dx.doi.org/10.1292/jvms.16-0340
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author SUZUKI, Kazuya
OGUMA, Keisuke
SENTSUI, Hiroshi
author_facet SUZUKI, Kazuya
OGUMA, Keisuke
SENTSUI, Hiroshi
author_sort SUZUKI, Kazuya
collection PubMed
description We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum.
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spelling pubmed-52892512017-02-08 Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens SUZUKI, Kazuya OGUMA, Keisuke SENTSUI, Hiroshi J Vet Med Sci Virology We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum. The Japanese Society of Veterinary Science 2016-10-30 2017-01 /pmc/articles/PMC5289251/ /pubmed/27795464 http://dx.doi.org/10.1292/jvms.16-0340 Text en ©2017 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
SUZUKI, Kazuya
OGUMA, Keisuke
SENTSUI, Hiroshi
Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title_full Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title_fullStr Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title_full_unstemmed Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title_short Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
title_sort preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289251/
https://www.ncbi.nlm.nih.gov/pubmed/27795464
http://dx.doi.org/10.1292/jvms.16-0340
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AT ogumakeisuke preparationofacelllinepersistentlyinfectedwithmaedivisnavirusandproductionofviralantigens
AT sentsuihiroshi preparationofacelllinepersistentlyinfectedwithmaedivisnavirusandproductionofviralantigens

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