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Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens

We attempted the isolation of variant infectious bursal disease (IBD) viruses by using sentinel chickens immunized with inactivated classical-type IBD vaccine. Immunized sentinel chickens with high levels of neutralizing antibodies and non-immunized sentinel chickens were raised together with broile...

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Autores principales: YAMAZAKI, Kenichi, OHTA, Hideyuki, KAWAI, Toru, YAMAGUCHI, Tsuyoshi, OBI, Takeshi, TAKASE, Kozo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289257/
https://www.ncbi.nlm.nih.gov/pubmed/27784860
http://dx.doi.org/10.1292/jvms.16-0301
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author YAMAZAKI, Kenichi
OHTA, Hideyuki
KAWAI, Toru
YAMAGUCHI, Tsuyoshi
OBI, Takeshi
TAKASE, Kozo
author_facet YAMAZAKI, Kenichi
OHTA, Hideyuki
KAWAI, Toru
YAMAGUCHI, Tsuyoshi
OBI, Takeshi
TAKASE, Kozo
author_sort YAMAZAKI, Kenichi
collection PubMed
description We attempted the isolation of variant infectious bursal disease (IBD) viruses by using sentinel chickens immunized with inactivated classical-type IBD vaccine. Immunized sentinel chickens with high levels of neutralizing antibodies and non-immunized sentinel chickens were raised together with broiler chickens in a commercial farm. Severe atrophy of the bursa of Fabricius was observed from the second week after cohabitation in non-immunized sentinel chickens. However, in immunized sentinel chickens and broiler chickens, atrophy was observed from the third week after cohabitation. The IBD virus (IBDV) isolated from the bursa of Fabricius of immunized sentinel chickens, designated as strain IBDV TY2, showed severe atrophy of the bursa in infected SPF chickens. Antiserum to the IBDV TY2 strain showed higher neutralizing activity to heterologous IBDV strains than did antiserum to the K strain vaccine virus. Phylogenetic analysis revealed that the nucleotide sequences encoding the hypervariable region of virus protein 2 of the IBDV TY2 strain did not cluster with the classical, variant or very virulent IBDV groups. Based on these results, we suggest that the IBDV TY2 strain may constitute a novel variant type of IBDV.
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spelling pubmed-52892572017-02-08 Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens YAMAZAKI, Kenichi OHTA, Hideyuki KAWAI, Toru YAMAGUCHI, Tsuyoshi OBI, Takeshi TAKASE, Kozo J Vet Med Sci Avian Pathology We attempted the isolation of variant infectious bursal disease (IBD) viruses by using sentinel chickens immunized with inactivated classical-type IBD vaccine. Immunized sentinel chickens with high levels of neutralizing antibodies and non-immunized sentinel chickens were raised together with broiler chickens in a commercial farm. Severe atrophy of the bursa of Fabricius was observed from the second week after cohabitation in non-immunized sentinel chickens. However, in immunized sentinel chickens and broiler chickens, atrophy was observed from the third week after cohabitation. The IBD virus (IBDV) isolated from the bursa of Fabricius of immunized sentinel chickens, designated as strain IBDV TY2, showed severe atrophy of the bursa in infected SPF chickens. Antiserum to the IBDV TY2 strain showed higher neutralizing activity to heterologous IBDV strains than did antiserum to the K strain vaccine virus. Phylogenetic analysis revealed that the nucleotide sequences encoding the hypervariable region of virus protein 2 of the IBDV TY2 strain did not cluster with the classical, variant or very virulent IBDV groups. Based on these results, we suggest that the IBDV TY2 strain may constitute a novel variant type of IBDV. The Japanese Society of Veterinary Science 2016-10-27 2017-01 /pmc/articles/PMC5289257/ /pubmed/27784860 http://dx.doi.org/10.1292/jvms.16-0301 Text en ©2017 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Avian Pathology
YAMAZAKI, Kenichi
OHTA, Hideyuki
KAWAI, Toru
YAMAGUCHI, Tsuyoshi
OBI, Takeshi
TAKASE, Kozo
Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title_full Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title_fullStr Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title_full_unstemmed Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title_short Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens
title_sort characterization of variant infectious bursal disease virus from a broiler farm in japan using immunized sentinel chickens
topic Avian Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289257/
https://www.ncbi.nlm.nih.gov/pubmed/27784860
http://dx.doi.org/10.1292/jvms.16-0301
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