Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts

Flavonoids are natural polyphenols that are widely found in plants. The effects of flavonoids on obesity and numerous diseases such as cancer, diabetes, and Alzheimer’s have been well studied. However, little is known about the relationships between flavonoids and the circadian clock. In this study,...

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Autores principales: Shinozaki, Ayako, Misawa, Kenichiro, Ikeda, Yuko, Haraguchi, Atsushi, Kamagata, Mayo, Tahara, Yu, Shibata, Shigenobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289493/
https://www.ncbi.nlm.nih.gov/pubmed/28152057
http://dx.doi.org/10.1371/journal.pone.0170904
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author Shinozaki, Ayako
Misawa, Kenichiro
Ikeda, Yuko
Haraguchi, Atsushi
Kamagata, Mayo
Tahara, Yu
Shibata, Shigenobu
author_facet Shinozaki, Ayako
Misawa, Kenichiro
Ikeda, Yuko
Haraguchi, Atsushi
Kamagata, Mayo
Tahara, Yu
Shibata, Shigenobu
author_sort Shinozaki, Ayako
collection PubMed
description Flavonoids are natural polyphenols that are widely found in plants. The effects of flavonoids on obesity and numerous diseases such as cancer, diabetes, and Alzheimer’s have been well studied. However, little is known about the relationships between flavonoids and the circadian clock. In this study, we show that continuous or transient application of flavonoids to the culture medium of embryonic fibroblasts from PER2::LUCIFERASE (PER2::LUC) mice induced various modifications in the circadian clock amplitude, period, and phase. Transient application of some of the tested flavonoids to cultured cells induced a phase delay of the PER2::LUC rhythm at the down slope phase. In addition, continuous application of the polymethoxy flavonoids nobiletin and tangeretin increased the amplitude and lengthened the period of the PER2::LUC rhythm. The nobiletin-induced phase delay was blocked by co-treatment with U0126, an ERK inhibitor. In summary, among the tested flavonoids, polymethoxy flavones increased the amplitude, lengthened the period, and delayed the phase of the PER2::LUC circadian rhythm. Therefore, foods that contain polymethoxy flavones may have beneficial effects on circadian rhythm disorders and jet lag.
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spelling pubmed-52894932017-02-17 Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts Shinozaki, Ayako Misawa, Kenichiro Ikeda, Yuko Haraguchi, Atsushi Kamagata, Mayo Tahara, Yu Shibata, Shigenobu PLoS One Research Article Flavonoids are natural polyphenols that are widely found in plants. The effects of flavonoids on obesity and numerous diseases such as cancer, diabetes, and Alzheimer’s have been well studied. However, little is known about the relationships between flavonoids and the circadian clock. In this study, we show that continuous or transient application of flavonoids to the culture medium of embryonic fibroblasts from PER2::LUCIFERASE (PER2::LUC) mice induced various modifications in the circadian clock amplitude, period, and phase. Transient application of some of the tested flavonoids to cultured cells induced a phase delay of the PER2::LUC rhythm at the down slope phase. In addition, continuous application of the polymethoxy flavonoids nobiletin and tangeretin increased the amplitude and lengthened the period of the PER2::LUC rhythm. The nobiletin-induced phase delay was blocked by co-treatment with U0126, an ERK inhibitor. In summary, among the tested flavonoids, polymethoxy flavones increased the amplitude, lengthened the period, and delayed the phase of the PER2::LUC circadian rhythm. Therefore, foods that contain polymethoxy flavones may have beneficial effects on circadian rhythm disorders and jet lag. Public Library of Science 2017-02-02 /pmc/articles/PMC5289493/ /pubmed/28152057 http://dx.doi.org/10.1371/journal.pone.0170904 Text en © 2017 Shinozaki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shinozaki, Ayako
Misawa, Kenichiro
Ikeda, Yuko
Haraguchi, Atsushi
Kamagata, Mayo
Tahara, Yu
Shibata, Shigenobu
Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title_full Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title_fullStr Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title_full_unstemmed Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title_short Potent Effects of Flavonoid Nobiletin on Amplitude, Period, and Phase of the Circadian Clock Rhythm in PER2::LUCIFERASE Mouse Embryonic Fibroblasts
title_sort potent effects of flavonoid nobiletin on amplitude, period, and phase of the circadian clock rhythm in per2::luciferase mouse embryonic fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289493/
https://www.ncbi.nlm.nih.gov/pubmed/28152057
http://dx.doi.org/10.1371/journal.pone.0170904
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