A putative multi-replicon plasmid co-harboring beta-lactamase genes bla(KPC-2), bla(CTX-M-14) and bla(TEM-1) and trimethoprim resistance gene dfrA25 from a Klebsiella pneumoniae sequence type (ST) 11 strain in China

The global emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae poses a major public health threat requiring immediate and aggressive action. Some older generation antibiotics, such as trimethoprim, serve as alternatives for treatment of infections. Here, we determined the complete nucleotide sequence of plasmid pHS091147, which co-harbored the carbapenemase (bla(KPC-2)) and trimethoprim resistance genes (dfrA25) from a Klebsiella pneumoniae sequence type (ST) 11 clone recovered in Shanghai, China. pHS091147 had three replication genes, several plasmid-stability genes and an intact type IV secretion system gene cluster. Besides bla(KPC-2) and dfrA25, pHS091147 carried several other resistance genes, including β-lactamase genes bla(TEM-1) and bla(CTX-M-14), sulphonamide resistance gene sul1, a quinolone resistance gene remnant (ΔqnrB2), and virulence associated gene iroN. Notably, the multidrug-resistance region was a chimeric structure composed of three subregions, which shared strong sequence homology with several plasmids previously assigned in Genbank. To our knowledge, this is the first report of the co-localization of bla(KPC-2) and dfrA25 on a novel putative multi-replicon plasmid in a Klebsiella pneumoniae ST11 clone.