Cargando…

Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP

Pseudokirchneriella subcapitata is a sickle-shaped freshwater green microalga that is normally found in unicellular form. Currently, it is the best known and most frequently used species of ecotoxicological bioindicator because of its high growth rate and sensitivity to toxicants. However, despite t...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamagishi, Takahiro, Yamaguchi, Haruyo, Suzuki, Shigekatsu, Horie, Yoshifumi, Tatarazako, Norihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289587/
https://www.ncbi.nlm.nih.gov/pubmed/28152022
http://dx.doi.org/10.1371/journal.pone.0171259
_version_ 1782504519678033920
author Yamagishi, Takahiro
Yamaguchi, Haruyo
Suzuki, Shigekatsu
Horie, Yoshifumi
Tatarazako, Norihisa
author_facet Yamagishi, Takahiro
Yamaguchi, Haruyo
Suzuki, Shigekatsu
Horie, Yoshifumi
Tatarazako, Norihisa
author_sort Yamagishi, Takahiro
collection PubMed
description Pseudokirchneriella subcapitata is a sickle-shaped freshwater green microalga that is normally found in unicellular form. Currently, it is the best known and most frequently used species of ecotoxicological bioindicator because of its high growth rate and sensitivity to toxicants. However, despite this organism’s, our knowledge of its cell biology—for example, the patterns of nuclear and cytoplasmic division in the mitotic stage—is limited. Although it has been reported that P. subcapitata proliferates by popularity forming four daughter cells (autospores) through multiple fission after two nuclear divisions, here, we report two additional reproductive patterns by which two autospores are formed by binary fission (“two-autospore type”) and eight autospores are formed by multiple fission (“eight-autospore type”). Moreover, we found that cell reproductive patterns differed markedly with the culture conditions or with exposure to either of two typical toxicants, potassium dichromate (K(2)Cr(2)O(7)) and 3,5-dichlorophenol (3,5-DCP). The eight-autospore type occurred at the highest frequency in the early phase of culture, but it disappeared under 3,5-DCP at 2.0 mg/L. Under 0.3 mg/L K(2)CrO(7) (Cr(VI)) the eight-autospore type took substantially longer to appear than in control culture. The two-autospore type occurred only in the late phase of culture. To our knowledge, this is the first detailed evaluation of the reproductive patterns of P. subcapitata, which changed dramatically in the presence of toxicants. These findings suggest that observation of the reproductive patterns of P. subcapitata will help to elucidate different cell reactions to toxicants.
format Online
Article
Text
id pubmed-5289587
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-52895872017-02-17 Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP Yamagishi, Takahiro Yamaguchi, Haruyo Suzuki, Shigekatsu Horie, Yoshifumi Tatarazako, Norihisa PLoS One Research Article Pseudokirchneriella subcapitata is a sickle-shaped freshwater green microalga that is normally found in unicellular form. Currently, it is the best known and most frequently used species of ecotoxicological bioindicator because of its high growth rate and sensitivity to toxicants. However, despite this organism’s, our knowledge of its cell biology—for example, the patterns of nuclear and cytoplasmic division in the mitotic stage—is limited. Although it has been reported that P. subcapitata proliferates by popularity forming four daughter cells (autospores) through multiple fission after two nuclear divisions, here, we report two additional reproductive patterns by which two autospores are formed by binary fission (“two-autospore type”) and eight autospores are formed by multiple fission (“eight-autospore type”). Moreover, we found that cell reproductive patterns differed markedly with the culture conditions or with exposure to either of two typical toxicants, potassium dichromate (K(2)Cr(2)O(7)) and 3,5-dichlorophenol (3,5-DCP). The eight-autospore type occurred at the highest frequency in the early phase of culture, but it disappeared under 3,5-DCP at 2.0 mg/L. Under 0.3 mg/L K(2)CrO(7) (Cr(VI)) the eight-autospore type took substantially longer to appear than in control culture. The two-autospore type occurred only in the late phase of culture. To our knowledge, this is the first detailed evaluation of the reproductive patterns of P. subcapitata, which changed dramatically in the presence of toxicants. These findings suggest that observation of the reproductive patterns of P. subcapitata will help to elucidate different cell reactions to toxicants. Public Library of Science 2017-02-02 /pmc/articles/PMC5289587/ /pubmed/28152022 http://dx.doi.org/10.1371/journal.pone.0171259 Text en © 2017 Yamagishi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yamagishi, Takahiro
Yamaguchi, Haruyo
Suzuki, Shigekatsu
Horie, Yoshifumi
Tatarazako, Norihisa
Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title_full Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title_fullStr Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title_full_unstemmed Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title_short Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP
title_sort cell reproductive patterns in the green alga pseudokirchneriella subcapitata (=selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-dcp
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289587/
https://www.ncbi.nlm.nih.gov/pubmed/28152022
http://dx.doi.org/10.1371/journal.pone.0171259
work_keys_str_mv AT yamagishitakahiro cellreproductivepatternsinthegreenalgapseudokirchneriellasubcapitataselenastrumcapricornutumandtheirvariationsunderexposuretothetypicaltoxicantspotassiumdichromateand35dcp
AT yamaguchiharuyo cellreproductivepatternsinthegreenalgapseudokirchneriellasubcapitataselenastrumcapricornutumandtheirvariationsunderexposuretothetypicaltoxicantspotassiumdichromateand35dcp
AT suzukishigekatsu cellreproductivepatternsinthegreenalgapseudokirchneriellasubcapitataselenastrumcapricornutumandtheirvariationsunderexposuretothetypicaltoxicantspotassiumdichromateand35dcp
AT horieyoshifumi cellreproductivepatternsinthegreenalgapseudokirchneriellasubcapitataselenastrumcapricornutumandtheirvariationsunderexposuretothetypicaltoxicantspotassiumdichromateand35dcp
AT tatarazakonorihisa cellreproductivepatternsinthegreenalgapseudokirchneriellasubcapitataselenastrumcapricornutumandtheirvariationsunderexposuretothetypicaltoxicantspotassiumdichromateand35dcp