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Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions
BACKGROUND: There is growing recognition of the significance of the gut microbiome to human health, and the association between a perturbed gut microbiome with human diseases has been established. Previous studies also show the role of environmental toxicants in perturbing the gut microbiome and its...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Environmental Health Sciences
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289904/ https://www.ncbi.nlm.nih.gov/pubmed/27203275 http://dx.doi.org/10.1289/EHP202 |
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author | Gao, Bei Bian, Xiaoming Mahbub, Ridwan Lu, Kun |
author_facet | Gao, Bei Bian, Xiaoming Mahbub, Ridwan Lu, Kun |
author_sort | Gao, Bei |
collection | PubMed |
description | BACKGROUND: There is growing recognition of the significance of the gut microbiome to human health, and the association between a perturbed gut microbiome with human diseases has been established. Previous studies also show the role of environmental toxicants in perturbing the gut microbiome and its metabolic functions. The wide agricultural use of diazinon, an organophosphate insecticide, has raised serious environmental health concerns since it is a potent neurotoxicant. With studies demonstrating the presence of a microbiome–gut–brain axis, it is possible that gut microbiome perturbation may also contribute to diazinon toxicity. OBJECTIVES: We investigated the impact of diazinon exposure on the gut microbiome composition and its metabolic functions in C57BL/6 mice. METHODS: We used a combination of 16S rRNA gene sequencing, metagenomics sequencing, and mass spectrometry–based metabolomics profiling in a mouse model to examine the functional impact of diazinon on the gut microbiome. RESULTS: 16S rRNA gene sequencing revealed that diazinon exposure significantly perturbed the gut microbiome, and metagenomic sequencing found that diazinon exposure altered the functional metagenome. Moreover, metabolomics profiling revealed an altered metabolic profile arising from exposure. Of particular significance, these changes were more pronounced for male mice than for female mice. CONCLUSIONS: Diazinon exposure perturbed the gut microbiome community structure, functional metagenome, and associated metabolic profiles in a sex-specific manner. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism contributing to diazinon neurotoxicity and, in particular, its sex-selective effects. CITATION: Gao B, Bian X, Mahbub R, Lu K. 2017. Sex-specific effects of organophosphate diazinon on the gut microbiome and its metabolic functions. Environ Health Perspect 125:198–206; http://dx.doi.org/10.1289/EHP202 |
format | Online Article Text |
id | pubmed-5289904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | National Institute of Environmental Health Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-52899042017-02-06 Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions Gao, Bei Bian, Xiaoming Mahbub, Ridwan Lu, Kun Environ Health Perspect Research BACKGROUND: There is growing recognition of the significance of the gut microbiome to human health, and the association between a perturbed gut microbiome with human diseases has been established. Previous studies also show the role of environmental toxicants in perturbing the gut microbiome and its metabolic functions. The wide agricultural use of diazinon, an organophosphate insecticide, has raised serious environmental health concerns since it is a potent neurotoxicant. With studies demonstrating the presence of a microbiome–gut–brain axis, it is possible that gut microbiome perturbation may also contribute to diazinon toxicity. OBJECTIVES: We investigated the impact of diazinon exposure on the gut microbiome composition and its metabolic functions in C57BL/6 mice. METHODS: We used a combination of 16S rRNA gene sequencing, metagenomics sequencing, and mass spectrometry–based metabolomics profiling in a mouse model to examine the functional impact of diazinon on the gut microbiome. RESULTS: 16S rRNA gene sequencing revealed that diazinon exposure significantly perturbed the gut microbiome, and metagenomic sequencing found that diazinon exposure altered the functional metagenome. Moreover, metabolomics profiling revealed an altered metabolic profile arising from exposure. Of particular significance, these changes were more pronounced for male mice than for female mice. CONCLUSIONS: Diazinon exposure perturbed the gut microbiome community structure, functional metagenome, and associated metabolic profiles in a sex-specific manner. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism contributing to diazinon neurotoxicity and, in particular, its sex-selective effects. CITATION: Gao B, Bian X, Mahbub R, Lu K. 2017. Sex-specific effects of organophosphate diazinon on the gut microbiome and its metabolic functions. Environ Health Perspect 125:198–206; http://dx.doi.org/10.1289/EHP202 National Institute of Environmental Health Sciences 2016-05-20 2017-02 /pmc/articles/PMC5289904/ /pubmed/27203275 http://dx.doi.org/10.1289/EHP202 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, “Reproduced with permission from Environmental Health Perspectives”); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright. |
spellingShingle | Research Gao, Bei Bian, Xiaoming Mahbub, Ridwan Lu, Kun Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title | Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title_full | Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title_fullStr | Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title_full_unstemmed | Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title_short | Sex-Specific Effects of Organophosphate Diazinon on the Gut Microbiome and Its Metabolic Functions |
title_sort | sex-specific effects of organophosphate diazinon on the gut microbiome and its metabolic functions |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289904/ https://www.ncbi.nlm.nih.gov/pubmed/27203275 http://dx.doi.org/10.1289/EHP202 |
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