MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems

Efficient generation of plants carrying mutations in multiple genes remains a challenge. Using two or more orthogonal CRISPR/Cas systems can generate plants with multi-gene mutations, but assembly of these systems requires a robust, high-capacity toolkit. Here, we describe MISSA 2.0 (multiple-round...

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Detalles Bibliográficos
Autores principales: Zhang, Hai-Yan, Wang, Xing-Hui, Dong, Li, Wang, Zhi-Ping, Liu, Bing, Lv, Jie, Xing, Hui-Li, Han, Chun-Yan, Wang, Xue-Chen, Chen, Qi-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290471/
https://www.ncbi.nlm.nih.gov/pubmed/28155921
http://dx.doi.org/10.1038/srep41993

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