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The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis

BACKGROUND: The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. In this study, we aimed to evaluate exercise capacity in mice fed with a high fat diet (HFD) for 3 days and investigate the molecular mechani...

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Autores principales: Xiao, Ya, Wang, Wanshan, Chen, Liguo, Chen, Jieyu, Jiang, Pingping, Fu, Xiuqiong, Nie, Xiaoli, Kwan, Hiuyee, Liu, Yanyan, Zhao, Xiaoshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290644/
https://www.ncbi.nlm.nih.gov/pubmed/28153015
http://dx.doi.org/10.1186/s12944-017-0424-7
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author Xiao, Ya
Wang, Wanshan
Chen, Liguo
Chen, Jieyu
Jiang, Pingping
Fu, Xiuqiong
Nie, Xiaoli
Kwan, Hiuyee
Liu, Yanyan
Zhao, Xiaoshan
author_facet Xiao, Ya
Wang, Wanshan
Chen, Liguo
Chen, Jieyu
Jiang, Pingping
Fu, Xiuqiong
Nie, Xiaoli
Kwan, Hiuyee
Liu, Yanyan
Zhao, Xiaoshan
author_sort Xiao, Ya
collection PubMed
description BACKGROUND: The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. In this study, we aimed to evaluate exercise capacity in mice fed with a high fat diet (HFD) for 3 days and investigate the molecular mechanisms in the early response to high-fat feeding. METHODS: Exercise capacity was assessed by weight-loaded swimming test in mice fed a control diet (10 kcal% fat) or a HFD (60 kcal% fat) for 3 days. Global gene expression of ten important tissues (brain, heart, liver, spleen, lung, kidney, stomach, duodenum, skeletal muscle and blood) was analyzed using RNA Sequencing. RESULTS: A HFD for just 3 days can induce 71% decrease of exercise performance prior to substantial weight gain (P <0.01). Principle component analysis revealed that differential gene expression patterns existed in the ten tissues. Out of which, the brain, spleen and lung were demonstrated to have more pronounced transcriptional changes than other tissues. Biological process analysis for differentially expressed genes in the brain, spleen and lung showed that dysregulation of peripheral and central immune response had been implicated in the early stage of HFD exposure. Neurotransmission related genes and circulatory system process related genes were significantly down-regulated in the brain and lung, respectively. CONCLUSIONS: Our findings provide new insights for the deleterious effects of high-fat feeding, especially revealing that the lung maybe as a new important target attacked by short-term high-fat feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0424-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-52906442017-02-07 The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis Xiao, Ya Wang, Wanshan Chen, Liguo Chen, Jieyu Jiang, Pingping Fu, Xiuqiong Nie, Xiaoli Kwan, Hiuyee Liu, Yanyan Zhao, Xiaoshan Lipids Health Dis Research BACKGROUND: The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. In this study, we aimed to evaluate exercise capacity in mice fed with a high fat diet (HFD) for 3 days and investigate the molecular mechanisms in the early response to high-fat feeding. METHODS: Exercise capacity was assessed by weight-loaded swimming test in mice fed a control diet (10 kcal% fat) or a HFD (60 kcal% fat) for 3 days. Global gene expression of ten important tissues (brain, heart, liver, spleen, lung, kidney, stomach, duodenum, skeletal muscle and blood) was analyzed using RNA Sequencing. RESULTS: A HFD for just 3 days can induce 71% decrease of exercise performance prior to substantial weight gain (P <0.01). Principle component analysis revealed that differential gene expression patterns existed in the ten tissues. Out of which, the brain, spleen and lung were demonstrated to have more pronounced transcriptional changes than other tissues. Biological process analysis for differentially expressed genes in the brain, spleen and lung showed that dysregulation of peripheral and central immune response had been implicated in the early stage of HFD exposure. Neurotransmission related genes and circulatory system process related genes were significantly down-regulated in the brain and lung, respectively. CONCLUSIONS: Our findings provide new insights for the deleterious effects of high-fat feeding, especially revealing that the lung maybe as a new important target attacked by short-term high-fat feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0424-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-02 /pmc/articles/PMC5290644/ /pubmed/28153015 http://dx.doi.org/10.1186/s12944-017-0424-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xiao, Ya
Wang, Wanshan
Chen, Liguo
Chen, Jieyu
Jiang, Pingping
Fu, Xiuqiong
Nie, Xiaoli
Kwan, Hiuyee
Liu, Yanyan
Zhao, Xiaoshan
The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title_full The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title_fullStr The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title_full_unstemmed The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title_short The effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by RNA sequencing analysis
title_sort effects of short-term high-fat feeding on exercise capacity: multi-tissue transcriptome changes by rna sequencing analysis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290644/
https://www.ncbi.nlm.nih.gov/pubmed/28153015
http://dx.doi.org/10.1186/s12944-017-0424-7
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