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The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism

BACKGROUND: Acetate is a common microbial fermentative end-product, which can potentially be used as a supplementary carbon source to enhance the output of biotechnological production systems. This study focuses on the acetate metabolism of the photosynthetic cyanobacterium Synechocystis sp. PCC 680...

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Autores principales: Thiel, Kati, Vuorio, Eerika, Aro, Eva-Mari, Kallio, Pauli Tapio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290672/
https://www.ncbi.nlm.nih.gov/pubmed/28153019
http://dx.doi.org/10.1186/s12934-017-0640-x
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author Thiel, Kati
Vuorio, Eerika
Aro, Eva-Mari
Kallio, Pauli Tapio
author_facet Thiel, Kati
Vuorio, Eerika
Aro, Eva-Mari
Kallio, Pauli Tapio
author_sort Thiel, Kati
collection PubMed
description BACKGROUND: Acetate is a common microbial fermentative end-product, which can potentially be used as a supplementary carbon source to enhance the output of biotechnological production systems. This study focuses on the acetate metabolism of the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 which is unable to grow on acetate as a sole carbon source but still can assimilate it via acetyl-CoA—derived metabolic intermediates. In order to gain insight into the acetate uptake, associated limitations and metabolic effects, a heterologous acetate transporter ActP from Escherichia coli was introduced into Synechocystis to facilitate the transport of supplemented acetate from the medium into the cell. RESULTS: The results show that enhanced acetate intake can efficiently promote the growth of the cyanobacterial host. The effect is apparent specifically under low-light conditions when the photosynthetic activity is low, and expected to result from increased availability of acetyl-CoA precursors, accompanied by changes induced in cellular glycogen metabolism which may include allocation of resources towards enhanced growth instead of glycogen accumulation. Despite the stimulated growth of the mutant, acetate is shown to suppress the activity of the photosynthetic apparatus, further emphasizing the contribution of glycolytic metabolism in the acetate-induced effect. CONCLUSIONS: The use of acetate by the cyanobacterium Synechocystis sp. PCC 6803 is at least partially restricted by the import into the cell. This can be improved by the introduction of a heterologous acetate transporter into the system, thereby providing a potential advantage by expanding the scope of acetate utilization for various biosynthetic processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-017-0640-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-52906722017-02-07 The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism Thiel, Kati Vuorio, Eerika Aro, Eva-Mari Kallio, Pauli Tapio Microb Cell Fact Research BACKGROUND: Acetate is a common microbial fermentative end-product, which can potentially be used as a supplementary carbon source to enhance the output of biotechnological production systems. This study focuses on the acetate metabolism of the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 which is unable to grow on acetate as a sole carbon source but still can assimilate it via acetyl-CoA—derived metabolic intermediates. In order to gain insight into the acetate uptake, associated limitations and metabolic effects, a heterologous acetate transporter ActP from Escherichia coli was introduced into Synechocystis to facilitate the transport of supplemented acetate from the medium into the cell. RESULTS: The results show that enhanced acetate intake can efficiently promote the growth of the cyanobacterial host. The effect is apparent specifically under low-light conditions when the photosynthetic activity is low, and expected to result from increased availability of acetyl-CoA precursors, accompanied by changes induced in cellular glycogen metabolism which may include allocation of resources towards enhanced growth instead of glycogen accumulation. Despite the stimulated growth of the mutant, acetate is shown to suppress the activity of the photosynthetic apparatus, further emphasizing the contribution of glycolytic metabolism in the acetate-induced effect. CONCLUSIONS: The use of acetate by the cyanobacterium Synechocystis sp. PCC 6803 is at least partially restricted by the import into the cell. This can be improved by the introduction of a heterologous acetate transporter into the system, thereby providing a potential advantage by expanding the scope of acetate utilization for various biosynthetic processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-017-0640-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-02 /pmc/articles/PMC5290672/ /pubmed/28153019 http://dx.doi.org/10.1186/s12934-017-0640-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Thiel, Kati
Vuorio, Eerika
Aro, Eva-Mari
Kallio, Pauli Tapio
The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title_full The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title_fullStr The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title_full_unstemmed The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title_short The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
title_sort effect of enhanced acetate influx on synechocystis sp. pcc 6803 metabolism
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290672/
https://www.ncbi.nlm.nih.gov/pubmed/28153019
http://dx.doi.org/10.1186/s12934-017-0640-x
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