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mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes

Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian targe...

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Autores principales: Yin, Geng, Liang, Yan, Wang, Ying, Yang, Yuan, Yang, Min, Cen, Xiao-min, Xie, Qi-bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291140/
https://www.ncbi.nlm.nih.gov/pubmed/28057888
http://dx.doi.org/10.1042/BSR20160479
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author Yin, Geng
Liang, Yan
Wang, Ying
Yang, Yuan
Yang, Min
Cen, Xiao-min
Xie, Qi-bing
author_facet Yin, Geng
Liang, Yan
Wang, Ying
Yang, Yuan
Yang, Min
Cen, Xiao-min
Xie, Qi-bing
author_sort Yin, Geng
collection PubMed
description Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian target of rapamycin (mTOR) plays a vital role in the sensing of extracellular stimulations and regulating intracellular adaptations. Here, we report that mTOR complex 1 (mTORC1) signalling is a central regulator of lipid homoeostasis in lymphocytes. In hypoxia, mTORC1 activity is reduced and shifts lipid synthesis to lipid oxidation. Moreover, knockdown tuberous sclerosis complex 1 (TSC1) constitutively activates mTORC1 activity and impairs the hypoxia-induced metabolic shift. Therefore, TSC1 knockdown enhances hypoxia-induced cell death. Re-inactivation of mTORC1 activity via rapamycin may resist hypoxia-induced cell death in TSC1 knockdown lymphocytes. Our findings provide a deep insight into mTORC1 in the metabolic balance of lipid synthesis and oxidation, and imply that mTORC1 activity should be precisely regulated for the lipid homoeostasis in lymphocytes.
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spelling pubmed-52911402017-02-28 mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes Yin, Geng Liang, Yan Wang, Ying Yang, Yuan Yang, Min Cen, Xiao-min Xie, Qi-bing Biosci Rep Research Articles Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian target of rapamycin (mTOR) plays a vital role in the sensing of extracellular stimulations and regulating intracellular adaptations. Here, we report that mTOR complex 1 (mTORC1) signalling is a central regulator of lipid homoeostasis in lymphocytes. In hypoxia, mTORC1 activity is reduced and shifts lipid synthesis to lipid oxidation. Moreover, knockdown tuberous sclerosis complex 1 (TSC1) constitutively activates mTORC1 activity and impairs the hypoxia-induced metabolic shift. Therefore, TSC1 knockdown enhances hypoxia-induced cell death. Re-inactivation of mTORC1 activity via rapamycin may resist hypoxia-induced cell death in TSC1 knockdown lymphocytes. Our findings provide a deep insight into mTORC1 in the metabolic balance of lipid synthesis and oxidation, and imply that mTORC1 activity should be precisely regulated for the lipid homoeostasis in lymphocytes. Portland Press Ltd. 2017-02-03 /pmc/articles/PMC5291140/ /pubmed/28057888 http://dx.doi.org/10.1042/BSR20160479 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Yin, Geng
Liang, Yan
Wang, Ying
Yang, Yuan
Yang, Min
Cen, Xiao-min
Xie, Qi-bing
mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title_full mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title_fullStr mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title_full_unstemmed mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title_short mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
title_sort mtor complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291140/
https://www.ncbi.nlm.nih.gov/pubmed/28057888
http://dx.doi.org/10.1042/BSR20160479
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