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mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes
Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian targe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291140/ https://www.ncbi.nlm.nih.gov/pubmed/28057888 http://dx.doi.org/10.1042/BSR20160479 |
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author | Yin, Geng Liang, Yan Wang, Ying Yang, Yuan Yang, Min Cen, Xiao-min Xie, Qi-bing |
author_facet | Yin, Geng Liang, Yan Wang, Ying Yang, Yuan Yang, Min Cen, Xiao-min Xie, Qi-bing |
author_sort | Yin, Geng |
collection | PubMed |
description | Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian target of rapamycin (mTOR) plays a vital role in the sensing of extracellular stimulations and regulating intracellular adaptations. Here, we report that mTOR complex 1 (mTORC1) signalling is a central regulator of lipid homoeostasis in lymphocytes. In hypoxia, mTORC1 activity is reduced and shifts lipid synthesis to lipid oxidation. Moreover, knockdown tuberous sclerosis complex 1 (TSC1) constitutively activates mTORC1 activity and impairs the hypoxia-induced metabolic shift. Therefore, TSC1 knockdown enhances hypoxia-induced cell death. Re-inactivation of mTORC1 activity via rapamycin may resist hypoxia-induced cell death in TSC1 knockdown lymphocytes. Our findings provide a deep insight into mTORC1 in the metabolic balance of lipid synthesis and oxidation, and imply that mTORC1 activity should be precisely regulated for the lipid homoeostasis in lymphocytes. |
format | Online Article Text |
id | pubmed-5291140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52911402017-02-28 mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes Yin, Geng Liang, Yan Wang, Ying Yang, Yuan Yang, Min Cen, Xiao-min Xie, Qi-bing Biosci Rep Research Articles Mammalian cells adapt to different environmental conditions and alter cellular metabolic pathways to meet the energy demand for survival. Thus, the metabolic regulation of cells under special conditions, such as hypoxia, should be precisely regulated. During the metabolic regulation, mammalian target of rapamycin (mTOR) plays a vital role in the sensing of extracellular stimulations and regulating intracellular adaptations. Here, we report that mTOR complex 1 (mTORC1) signalling is a central regulator of lipid homoeostasis in lymphocytes. In hypoxia, mTORC1 activity is reduced and shifts lipid synthesis to lipid oxidation. Moreover, knockdown tuberous sclerosis complex 1 (TSC1) constitutively activates mTORC1 activity and impairs the hypoxia-induced metabolic shift. Therefore, TSC1 knockdown enhances hypoxia-induced cell death. Re-inactivation of mTORC1 activity via rapamycin may resist hypoxia-induced cell death in TSC1 knockdown lymphocytes. Our findings provide a deep insight into mTORC1 in the metabolic balance of lipid synthesis and oxidation, and imply that mTORC1 activity should be precisely regulated for the lipid homoeostasis in lymphocytes. Portland Press Ltd. 2017-02-03 /pmc/articles/PMC5291140/ /pubmed/28057888 http://dx.doi.org/10.1042/BSR20160479 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Yin, Geng Liang, Yan Wang, Ying Yang, Yuan Yang, Min Cen, Xiao-min Xie, Qi-bing mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title | mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title_full | mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title_fullStr | mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title_full_unstemmed | mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title_short | mTOR complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
title_sort | mtor complex 1 signalling regulates the balance between lipid synthesis and oxidation in hypoxia lymphocytes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291140/ https://www.ncbi.nlm.nih.gov/pubmed/28057888 http://dx.doi.org/10.1042/BSR20160479 |
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