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Conserved gene regulation during acute inflammation between zebrafish and mammals
Zebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases. However, in a context where even mice have been questioned as a valid model for these analysis, a systematic study evaluating the re...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291205/ https://www.ncbi.nlm.nih.gov/pubmed/28157230 http://dx.doi.org/10.1038/srep41905 |
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author | Forn-Cuní, G. Varela, M. Pereiro, P. Novoa, B. Figueras, A. |
author_facet | Forn-Cuní, G. Varela, M. Pereiro, P. Novoa, B. Figueras, A. |
author_sort | Forn-Cuní, G. |
collection | PubMed |
description | Zebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases. However, in a context where even mice have been questioned as a valid model for these analysis, a systematic study evaluating the reproducibility of human and mammalian inflammatory diseases in zebrafish is still lacking. In this report, we characterize the transcriptomic regulation to lipopolysaccharide in adult zebrafish kidney, liver, and muscle tissues using microarrays and demonstrate how the zebrafish genomic responses can effectively reproduce the mammalian inflammatory process induced by acute endotoxin stress. We provide evidence that immune signaling pathways and single gene expression is well conserved throughout evolution and that the zebrafish and mammal acute genomic responses after lipopolysaccharide stimulation are highly correlated despite the differential susceptibility between species to that compound. Therefore, we formally confirm that zebrafish inflammatory models are suited to study the basic mechanisms of inflammation in human inflammatory diseases, with great translational impact potential. |
format | Online Article Text |
id | pubmed-5291205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52912052017-02-07 Conserved gene regulation during acute inflammation between zebrafish and mammals Forn-Cuní, G. Varela, M. Pereiro, P. Novoa, B. Figueras, A. Sci Rep Article Zebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases. However, in a context where even mice have been questioned as a valid model for these analysis, a systematic study evaluating the reproducibility of human and mammalian inflammatory diseases in zebrafish is still lacking. In this report, we characterize the transcriptomic regulation to lipopolysaccharide in adult zebrafish kidney, liver, and muscle tissues using microarrays and demonstrate how the zebrafish genomic responses can effectively reproduce the mammalian inflammatory process induced by acute endotoxin stress. We provide evidence that immune signaling pathways and single gene expression is well conserved throughout evolution and that the zebrafish and mammal acute genomic responses after lipopolysaccharide stimulation are highly correlated despite the differential susceptibility between species to that compound. Therefore, we formally confirm that zebrafish inflammatory models are suited to study the basic mechanisms of inflammation in human inflammatory diseases, with great translational impact potential. Nature Publishing Group 2017-02-03 /pmc/articles/PMC5291205/ /pubmed/28157230 http://dx.doi.org/10.1038/srep41905 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Forn-Cuní, G. Varela, M. Pereiro, P. Novoa, B. Figueras, A. Conserved gene regulation during acute inflammation between zebrafish and mammals |
title | Conserved gene regulation during acute inflammation between zebrafish and mammals |
title_full | Conserved gene regulation during acute inflammation between zebrafish and mammals |
title_fullStr | Conserved gene regulation during acute inflammation between zebrafish and mammals |
title_full_unstemmed | Conserved gene regulation during acute inflammation between zebrafish and mammals |
title_short | Conserved gene regulation during acute inflammation between zebrafish and mammals |
title_sort | conserved gene regulation during acute inflammation between zebrafish and mammals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291205/ https://www.ncbi.nlm.nih.gov/pubmed/28157230 http://dx.doi.org/10.1038/srep41905 |
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