Sensitive and specific miRNA detection method using SplintR Ligase

We describe a simple, specific and sensitive microRNA (miRNA) detection method that utilizes Chlorella virus DNA ligase (SplintR(®) Ligase). This two-step method involves ligation of adjacent DNA oligonucleotides hybridized to a miRNA followed by real-time quantitative PCR (qPCR). SplintR Ligase is...

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Detalles Bibliográficos
Autores principales: Jin, Jingmin, Vaud, Sophie, Zhelkovsky, Alexander M., Posfai, Janos, McReynolds, Larry A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291259/
https://www.ncbi.nlm.nih.gov/pubmed/27154271
http://dx.doi.org/10.1093/nar/gkw399

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291259/
https://www.ncbi.nlm.nih.gov/pubmed/27154271
http://dx.doi.org/10.1093/nar/gkw399

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