Cargando…

Sensitive and specific miRNA detection method using SplintR Ligase

We describe a simple, specific and sensitive microRNA (miRNA) detection method that utilizes Chlorella virus DNA ligase (SplintR(®) Ligase). This two-step method involves ligation of adjacent DNA oligonucleotides hybridized to a miRNA followed by real-time quantitative PCR (qPCR). SplintR Ligase is...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jin, Jingmin, Vaud, Sophie, Zhelkovsky, Alexander M., Posfai, Janos, McReynolds, Larry A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291259/ https://www.ncbi.nlm.nih.gov/pubmed/27154271 http://dx.doi.org/10.1093/nar/gkw399

Ejemplares similares

Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase
por: Zhelkovsky, Alexander M., et al.
Publicado: (2011)

Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR
por: Qin, Xinshu, et al.
Publicado: (2022)

Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme
por: Zhelkovsky, Alexander M, et al.
Publicado: (2012)

Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand
por: Chen, Hui, et al.
Publicado: (2020)

A low-bias and sensitive small RNA library preparation method using randomized splint ligation
por: Maguire, Sean, et al.
Publicado: (2020)

Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals
por: Atanasov, Janina, et al.
Publicado: (2017)

miRdentify: high stringency miRNA predictor identifies several novel animal miRNAs
por: Hansen, Thomas B., et al.
Publicado: (2014)

miRNA target enrichment analysis reveals directly active miRNAs in health and disease
por: Steinfeld, Israel, et al.
Publicado: (2013)

Polysome shift assay for direct measurement of miRNA inhibition by anti-miRNA drugs
por: Androsavich, John R., et al.
Publicado: (2016)

Impact of probe annotation on the integration of miRNA–mRNA expression profiles for miRNA target detection
por: Sales, Gabriele, et al.
Publicado: (2010)

Wiring miRNAs to pathways: a topological approach to integrate miRNA and mRNA expression profiles
por: Calura, Enrica, et al.
Publicado: (2014)

Using shotgun sequence data to find active restriction enzyme genes
por: Zheng, Yu, et al.
Publicado: (2009)

miSTAR: miRNA target prediction through modeling quantitative and qualitative miRNA binding site information in a stacked model structure
por: Van Peer, Gert, et al.
Publicado: (2017)

miRVaS: a tool to predict the impact of genetic variants on miRNAs
por: Cammaerts, Sophia, et al.
Publicado: (2016)

Integrative analysis of gene and miRNA expression profiles with transcription factor–miRNA feed-forward loops identifies regulators in human cancers
por: Yan, Zhenyu, et al.
Publicado: (2012)

ncOrtho: efficient and reliable identification of miRNA orthologs
por: Langschied, Felix, et al.
Publicado: (2023)

miRspring: a compact standalone research tool for analyzing miRNA-seq data
por: Humphreys, David T., et al.
Publicado: (2013)

Comparative analysis of the end-joining activity of several DNA ligases
por: Bauer, Robert J., et al.
Publicado: (2017)

SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
por: Raine, Amanda, et al.
Publicado: (2017)

Lentivirus-mediated antagomir expression for specific inhibition of miRNA function
por: Scherr, Michaela, et al.
Publicado: (2007)

Prioritizing and selecting likely novel miRNAs from NGS data
por: Backes, Christina, et al.
Publicado: (2016)

Multiplexed miRNA northern blots via hybridization chain reaction
por: Schwarzkopf, Maayan, et al.
Publicado: (2016)

sPARTA: a parallelized pipeline for integrated analysis of plant miRNA and cleaved mRNA data sets, including new miRNA target-identification software
por: Kakrana, Atul, et al.
Publicado: (2014)

Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)
por: Braun, Juliane, et al.
Publicado: (2014)

A fast ab-initio method for predicting miRNA precursors in genomes
por: Tempel, Sébastien, et al.
Publicado: (2012)

Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
por: Nonne, Nora, et al.
Publicado: (2010)

Identification of active miRNA promoters from nuclear run-on RNA sequencing
por: Liu, Qi, et al.
Publicado: (2017)

Identification of miRNA targets with stable isotope labeling by amino acids in cell culture
por: Vinther, Jeppe, et al.
Publicado: (2006)

A high throughput experimental approach to identify miRNA targets in human cells
por: Tan, Lu Ping, et al.
Publicado: (2009)

Heterogeneous ensemble approach with discriminative features and modified-SMOTEbagging for pre-miRNA classification
por: Lertampaiporn, Supatcha, et al.
Publicado: (2013)

3′LIFE: a functional assay to detect miRNA targets in high-throughput
por: Wolter, Justin M., et al.
Publicado: (2014)

RILES, a novel method for temporal analysis of the in vivo regulation of miRNA expression
por: Ezzine, Safia, et al.
Publicado: (2013)

Quantitative analysis of conditional gene inactivation using rationally designed, tetracycline-controlled miRNAs
por: Berger, Stefan M., et al.
Publicado: (2010)

Profiling the selectivity of DNA ligases in an array format with mass spectrometry
por: Kim, Joohoon, et al.
Publicado: (2010)

Inferring probabilistic miRNA–mRNA interaction signatures in cancers: a role-switch approach
por: Li, Yue, et al.
Publicado: (2014)

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

Structural bias in T4 RNA ligase-mediated 3′-adapter ligation
por: Zhuang, Fanglei, et al.
Publicado: (2012)

A high-throughput assay for the comprehensive profiling of DNA ligase fidelity
por: Lohman, Gregory J. S., et al.
Publicado: (2016)

Discovery and visualization of miRNA–mRNA functional modules within integrated data using bicluster analysis
por: Bryan, Kenneth, et al.
Publicado: (2014)

TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus
por: Senís, Elena, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_kiop967fda4p3occ3sfg4iuedj