Cargando…

Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker

Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fou...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Jeahyuk, Park, Euiho, Lee, Se-Weon, Hyun, Jae-Wook, Baek, Kwang-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291402/
https://www.ncbi.nlm.nih.gov/pubmed/28167892
http://dx.doi.org/10.5423/PPJ.NT.09.2015.0188
_version_ 1782504771590029312
author Choi, Jeahyuk
Park, Euiho
Lee, Se-Weon
Hyun, Jae-Wook
Baek, Kwang-Hyun
author_facet Choi, Jeahyuk
Park, Euiho
Lee, Se-Weon
Hyun, Jae-Wook
Baek, Kwang-Hyun
author_sort Choi, Jeahyuk
collection PubMed
description Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fourteen hexapeptides were selected by using positional scanning of synthetic peptide combinatorial libraries. Each hexapeptide showed different antimicrobial spectrum against Bacillus, Pseudomonas, Xanthomonas, and Candida species. Intriguingly, BHC10 showed bactericidal activity exclusively on Xanthomonas citri subsp. citri (Xcc), while BHC7 was none-active exclusively against two Pseudomonas spp. at concentration of 100 μg/ml suggesting potential selectivity constrained in hexapeptide frame. Three hexapeptides, BHC02, 06 and 11, showed bactericidal activities against various Xcc strains at concentration of 10 μg/ml. When they were co-infiltrated with pathogens into citrus leaves the disease progress was suppressed significantly. Further study would be needed to confirm the actual disease control capacity of the selected hexapeptides.
format Online
Article
Text
id pubmed-5291402
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Korean Society of Plant Pathology
record_format MEDLINE/PubMed
spelling pubmed-52914022017-02-06 Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker Choi, Jeahyuk Park, Euiho Lee, Se-Weon Hyun, Jae-Wook Baek, Kwang-Hyun Plant Pathol J Note Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fourteen hexapeptides were selected by using positional scanning of synthetic peptide combinatorial libraries. Each hexapeptide showed different antimicrobial spectrum against Bacillus, Pseudomonas, Xanthomonas, and Candida species. Intriguingly, BHC10 showed bactericidal activity exclusively on Xanthomonas citri subsp. citri (Xcc), while BHC7 was none-active exclusively against two Pseudomonas spp. at concentration of 100 μg/ml suggesting potential selectivity constrained in hexapeptide frame. Three hexapeptides, BHC02, 06 and 11, showed bactericidal activities against various Xcc strains at concentration of 10 μg/ml. When they were co-infiltrated with pathogens into citrus leaves the disease progress was suppressed significantly. Further study would be needed to confirm the actual disease control capacity of the selected hexapeptides. Korean Society of Plant Pathology 2017-02 2017-02-01 /pmc/articles/PMC5291402/ /pubmed/28167892 http://dx.doi.org/10.5423/PPJ.NT.09.2015.0188 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Choi, Jeahyuk
Park, Euiho
Lee, Se-Weon
Hyun, Jae-Wook
Baek, Kwang-Hyun
Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title_full Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title_fullStr Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title_full_unstemmed Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title_short Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker
title_sort selection of small synthetic antimicrobial peptides inhibiting xanthomonas citri subsp. citri causing citrus canker
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291402/
https://www.ncbi.nlm.nih.gov/pubmed/28167892
http://dx.doi.org/10.5423/PPJ.NT.09.2015.0188
work_keys_str_mv AT choijeahyuk selectionofsmallsyntheticantimicrobialpeptidesinhibitingxanthomonascitrisubspcitricausingcitruscanker
AT parkeuiho selectionofsmallsyntheticantimicrobialpeptidesinhibitingxanthomonascitrisubspcitricausingcitruscanker
AT leeseweon selectionofsmallsyntheticantimicrobialpeptidesinhibitingxanthomonascitrisubspcitricausingcitruscanker
AT hyunjaewook selectionofsmallsyntheticantimicrobialpeptidesinhibitingxanthomonascitrisubspcitricausingcitruscanker
AT baekkwanghyun selectionofsmallsyntheticantimicrobialpeptidesinhibitingxanthomonascitrisubspcitricausingcitruscanker